超高效液相色谱-串联质谱法同时测定强肾排毒胶囊5种成分含量  

Simultaneous Determination of Five Components in Qiangshenpaidu Capsules by UPLCMS / MS

作　　者：蒋志涛 戴国梁[2] 潘金火[1,3] 王建春 陈晓峰 余辉 居文政[2] 

机构地区：[1]江苏省张家港市中医医院药学部江苏省企业研究生工作站,张家港215600 [2]南京中医药大学附属医院临床药理科,南京210029 [3]南京中医药大学药学院,南京210023

出　　处：《医药导报》2016年第7期772-775,共4页Herald of Medicine

基　　金：江苏省中医药领军人才培养项目(LJ200906);张家港市中医医院青年自然科学基金项目(zzyq1308)

摘　　要：目的建立超高效液相色谱-串联质谱法同时测定强肾排毒胶囊中5种成分腺苷、胞苷、鸟苷、甘露醇、腺嘌呤的含量。方法采用Agilent ZOBAX SB-C18(2.1 mm×150 mm,5μm)色谱柱;流动相为甲醇-0.1%甲酸溶液(5∶95),流速为0.2 m L·min^(-1);通过电喷雾离子源,选择正离子模式,多反应监测方式检测。结果腺苷、胞苷、鸟苷、甘露醇、腺嘌呤的线性范围分别为35~1 120 ng·m L^(-1)(r=0.998 1),10~320 ng·m L^(-1)(r=0.996 2),30~980 ng·m L^(-1)(r=0.999 3),40~1 280 ng·m L^(-1)(r=0.993 4),25~800 ng·m L^(-1)(r=0.992 5),加样回收率在97.4%~103.6%范围内,RSD均低于4.7%。结论所建立的方法专属性强、快速灵敏,可用于强肾排毒胶囊中腺苷、胞苷、鸟苷、甘露醇、腺嘌呤的含量测定。Objective To develop an UPLC-MS/MS method for simultaneously determination of five components （adenosine,cytidine,guanosine,mannitol and adenine） in Qiangshenpaidu capsules. Methods The UPLC separation was performed on an Agilent ZOBAX SB-C18（2.1 mm×150 mm,5 μm） column.Isocratic elution was carried out with mobile phase consisting of methanol-0.1%formic acid （5∶95） at a flow rate of 0.2 mL.min-1.The mass spectrometer was operated in the positive ionization electrospray （ ESI） mode using multiple monitoring （ MRM） for analysis of five components. Results Adenosine,cytidine,guanosine,mannitol and adenine were all analyzed with good precision and accuracy. The linear ranges were 35-1 120 ng.mL-1（ r=0.998 1） ,10-320 ng.mL-1（ r=0.996 4） , 30-980 ng.mL-1（ r=0.999 3） , 40-1 280 ng.mL-1（ r=0.993 4）, 25-800 ng.mL-1（r=0.996 5）,respectively.The recoveries of six analytes ranged from 97.4% to 103.6% and the relative standard deviations were all below 4.7%. Conclusion A sensitive,accurate and suitable UPLC-MS/MS method has been developed,and the method could be applied for the determination of adenosine,cytidine,guanosine,mannitol,and adenine in Qiangshenpaidu Capsules.

关 键 词：强肾排毒胶囊 超高效液相色谱-串联质谱法 腺苷 胞苷 鸟苷 甘露醇 腺嘌呤 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]