海金护卫散质量标准研究  

Study on Quality Standard of Haijinhuwei Powder

作　　者：张晓娟[1,2] 郭琪[2] 申睿[2] 杨莹[2] 李永申[2] 

机构地区：[1]河北北方学院,张家口075000 [2]北京军区联勤部药品仪器检验所,北京100071

出　　处：《医药导报》2016年第7期776-779,共4页Herald of Medicine

基　　金：军队医疗机构制剂标准提高科研专项课题(13ZJZ12-3)

摘　　要：目的：建立海金护卫散的质量标准。方法通过薄层色谱法对处方中的延胡索、白芷、当归分别进行定性鉴别；采用高效液相色谱法对延胡索中的延胡索乙素进行含量测定。结果薄层色谱斑点清晰,专属性强,阴性对照无干扰。含量测定中延胡索乙素在10.78~107.8μg.mL－1浓度范围内线性关系良好（r＝0.9999）,平均加样回收率96.57％（ n＝6）,RSD为1.40％（ n＝6）。结论建立的方法可靠,能准确进行定性、定量检测,可有效控制海金护卫散的质量。Objective To establish the quality standard for Haijinhuwei powder. Methods Corydalis Rhizoma, Angelicae Dahuricae Radix, Angelicae Sinensis Radix were identified by TLC. The content of tetrahydropalmatine was determined by HPLC. Results TLC spots were clear and specific.There was a good linear relationship between peak area and concentration of tetrahydropalmatine at the range of 10.78-107.8μg.mL-1（r=0.999 9）.The average recovery rate was 96.57% and RSD was 1.40%（n=6）. Conclusion All these results indicated that the developed TLC-HPLC method was proved to be reliable, accurate and specific, which could be used for the quality control of Haijinhuwei powder.

关 键 词：海金护卫散 延胡索乙素 色谱 薄层 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]