银杏总黄酮通过调节自噬减轻大鼠心肌缺血再灌注损伤的研究  

Yinxing leaf total flavonoid mitigates myocardial ischemia and reperfusi on injury via regulating autophagy

作　　者：沈秀珍 王凌 方毅华 

机构地区：[1]赤壁市人民医院心内科,湖北赤壁437300

出　　处：《安徽医药》2016年第6期1065-1067,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨银杏总黄酮是否可以通过调节自噬减轻心肌缺血再灌注(I/R)损伤以及可能的机制。方法采用大鼠心肌I/R模型(缺血30 min,再灌注4 h),雄性SD大鼠30只随机分为三组:假手术组(SO组,n=10)、缺血再灌注组(I/R组,n=10)和银杏总黄酮治疗组(YLTF+I/R组,n=10,100 mg·kg^(-1),造模前灌胃7 d)。检测心肌损伤标记物肌酸激酶(CK)、乳酸脱氢酶(LDH)和肌钙蛋白I(c Tn I);检测氧化应激水平,即丙二醛(MDA)和超氧化物歧化酶(SOD)水平;检测自噬蛋白beclin^(-1)和LC3水平。结果与SO组比较,I/R可诱导心肌损伤标记物(CK、LDH和c Tn I)表达升高,氧化应激水平的增加(MDA表达增加,SOD表达降低)和自噬的过度激活(LC3-Ⅱ/Ⅰ比值增加,beclin^(-1)表达增加);与I/R比较,银杏总黄酮可显著的降低心肌损伤标记物(CK、LDH和c Tn I)的表达、氧化应激水平(MDA表达降低,SOD表达增加)和自噬的过度激活(LC3-Ⅱ/Ⅰ比值降低,beclin^(-1)表达减低)。结论银杏总黄酮可以通过抑制过度自噬减轻心肌I/R损伤,这可能与其抑制氧化应激作用有关。Objective To investigate whether Yinxing leaf total flavonoid could mitigate myocardial ischemia and reperfusion（I /R）in-jury and the possible mechanism.Methods Rats I /R model （ischemia 30 min,then reperfusion 4 h）was used.30 rats were divided into three groups：sham -operation group （SO,n =10）,ischemia and reperfusion group （I /R,n =10）and Yinxing leaf total flavonoid treatment group （YLTF ＋I /R,n =10,100 mg·kg -1 ）.Cardiac injury markers （serum creatine（CK）,lactate dehydrogenase（LDH）, and cTnI）,oxidative stress （malondialdehyde （MDA）and superoxide dismutase （SOD））and autophagy markers （beclin-1 and LC3） were detected.Results Compared with SO group,levels of cardiac injury markers （CK,LDH and cTnI）and oxidative stress （MDA and SOD）significantly increased;autophagy was excessively activated in I /R group.Compared with I /R group,Yinxing leaf total fla-vonoid significantly decreased cardiac injury markers＇（CK,LDH and cTnI）expression and oxidative stress （MDA expression increased and SOD expression decreased）,and suppressed excessive activation of autophagy both beclin-1 level and LC3-Ⅱ/Ⅰ ratio decreased）. Conclusions Yinxing leaf total flavonoid could mitigate I /R injury via inhibiting excessive activation of autophagy.It＇s possible that Yinxing leaf total flavonoid inhibited excessive autophagy via suppressing oxidative stress.

关 键 词：再灌注损伤 银杏素 自噬 大鼠 Sprague-dawley 

分 类 号：R285.5[医药卫生—中药学]