羊传染性脓疱病毒疫苗株和野毒株VIR基因的比较分析  

Comparison and analysis of VIR gene from orf virus wild strain and attenuated vaccine strain

作　　者：王玲玲[1] 刘永杰[1] 郑海学[1] 张克山[1] 刘湘涛[1] 

机构地区：[1]中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/国家口蹄疫参考实验室,兰州730046

出　　处：《中国兽医学报》2016年第7期1135-1139,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31201914);中国博士后科学基金资助项目(2013M530683);国家现代肉羊产业技术体系项目(CARS-39)

摘　　要：羊传染性脓疱(Orf)是由羊传染性脓疱病毒(orf virus,ORFV)感染引起的一种人兽共患传染病。VIR是ORFV编码的抗干扰素基因。为比较分析ORFV疫苗弱毒株和野毒株编码的VIR基因的特征,本试验扩增并测定了ORFV疫苗株和野毒株的VIR基因序列,比较分析了两者核酸水平和氨基酸水平的变异情况以及二、三级蛋白结构。结果表明:本次测定的ORFV疫苗株与野毒VIR基因核苷酸序列相似性为94.6%,氨基酸序列相似性为91.8%,两者在Z-DNA结合结构域和ds-RNA结合结构域均有突变。Orf is an infectious zoonosis caused by orf virus（ORFV）.VIR is a virus interferon resistance gene of ORFV.In order to analyze the different characters between VIR genes from ORFV wild strain and attenuated vaccine strain,we amplified and sequenced nucleotide sequence of the two VIR genes,compared the nucleotide sequences and amino acid sequences,and predicted the secondary and tertiary structure.The results show that VIR gene from ORFV wild strain and vaccine strain shared 94.6%identity at nucleotide level and 91.8%identity at amino acid level.The difference between them occurred on both Z-DNA binding domain and ds-RNA binding domain.This result contributes to exploring the mechanism of ORFV attenuated.

关 键 词：羊口疮病毒 疫苗株 野毒株 VIR基因 比较分析 

分 类 号：S852.65[农业科学—基础兽医学]