济宁青山羊生后乳腺GHR和IGF-ⅠR的分布及其mRNA表达的发育性变化  

Location of GHR and IGF-ⅠR and their mRNA expressions in mammary gland of Jining gray goat during postnatal development

作　　者：常新侠 王树迎[1] 于学浩[1] 黄丽波[1] 侯衍猛[1] 

机构地区：[1]山东农业大学动物科技学院/山东省动物生物技术与疫病防治重点实验室,山东泰安271018

出　　处：《中国兽医学报》2016年第7期1234-1240,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30871832);山东省自然科学基金资助项目(ZR2009DM027);山东省科技攻关项目资助项目(2008GG30008018)

摘　　要：采用SABC法检测济宁青山羊生后发育过程中生长激素受体(growth hormone receptor,GHR)与胰岛素样生长因子Ⅰ受体(insulin like growth factorⅠreceptor,IGF-ⅠR)在乳腺组织中的分布;GAPDH作为内参基因,qRT-PCR法检测GHR mRNA与IGF-ⅠR mRNA相对表达量。结果显示:GHR和IGF-ⅠR阳性物质主要在乳腺组织内的导管上皮细胞、基质细胞和血管内皮细胞的胞质中表达,细胞核偶有表达;GHR和IGF-ⅠR阳性细胞光密度值与其mRNA表达趋势基本一致,但IGF-ⅠR的表达量低于GHR;GHR和IGF-ⅠR在出生当天表达水平较低,60日龄(初情期)和120日龄(性成熟)出现峰值(P<0.05),120日龄后逐渐下降。结果表明:济宁青山羊生后发育时期,GHR与IGF-ⅠR对乳腺的生长发育具有重要的调节作用,初情期和性成熟期可能是乳腺生长发育最快的阶段。To explore the localization and distribution of GHR and IGF-ⅠR as well as the expression of GHR mRNA and IGF-ⅠR mRNA in the mammary gland of Jining gray goat during postnatal development.The SABC method was employed to detect the localization of GHR and IGF-ⅠR in the mammary gland.GAPDH as the internal gene,the qRT-PCR was utilized to analyze the relative expression of GHR mRNA and IGF-ⅠR mRNA.GHR and IGF-ⅠR had similar variations,and they were mainly localized in the cytoplasmic of vascular endothelium,stromal cells and ductular epithelial cells of the mammary gland,occasionally in the nuclei.The changed trends of the optical density on the positive cells of GHR and IGF-ⅠR were similar to that of the expression of GHR mRNA and IGF-ⅠR mRNA,and the level of IGF-ⅠR was less than GHR.GHR and IGF-ⅠR were a lower expression level at the birth day,GHR and IGF-ⅠR reached their peak at the60days（puberty）and the 120days（sexual maturity）（P〈0.05）;and then they gradually decreased.GHR and IGF-ⅠR have an important effect on the growth of the mammary gland,and the puberty and the sexual maturity may be the fastest stage of the mammary gland during postnatal development in Jining gray goat.

关 键 词：生长激素受体 胰岛素样生长因子-Ⅰ受体 济宁青山羊 乳腺 免疫组化 QRT-PCR 

分 类 号：S814[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]