提高茸鹿冻精质量关键技术  

Key technology of improving the quality of frozen semen in deer

作　　者：韩欢胜[1,2] 赵列平[2] 姜昊[3] 魏巍[4] 柴孟龙[2] 张嘉保[3] 高利[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]黑龙江省农垦科学院哈尔滨特产研究所,黑龙江哈尔滨150038 [3]吉林大学动物科学学院,吉林长春130062 [4]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《中国兽医学报》2016年第7期1247-1250,共4页Chinese Journal of Veterinary Science

基　　金：黑龙江省应用技术研究与开发计划项目(GC13B407);黑龙江省农垦总局攻关项目(HNKIV-08-12A;HNK125B-12-05)

摘　　要：为进一步提高茸鹿冻精质量,对影响精液冷冻效果的关键因素进行了试验。结果评估得出：精液冷冻宜采用两步冷冻法,细管距液氮面2~3cm,冷冻时间5~7min;冷冻稀释液优先顺序为Trilady〉TrisB〉TrisA,Trilady稀释液从经济适用和冷冻效果上看最优;鲜精质量与冷冻效果密切相关,鲜精活力与冻存复苏率、解冻后精子活力呈正相关,R2分别为0.630 6和0.990 2;平衡时间在4℃宜平衡2~3h;平衡方法宜采用水浴中逐渐降温的方法。结果表明：通过进一步优化精液冷冻关键影响因素,可明显提高茸鹿冻精质量。To further improving the quality of frozen semen in deer,this test studied the key affecting factors about semen freezing.The assessment results showed that semen freezing way should adopt a two-step freezing method,Thin tubes should be put 2-3cm away from the plane of liquid nitrogen,Freezing time should be 5to 7min;the priority order of diluent to semen freezing was Trilady,TrisB and TrisA,Trilady is one of the most optimal in cost and frozen effect.Fresh semen quality and frozen effect was closely related,fresh sperm motility was positively correlated to the recovery rate of frozen semen,thawed sperm motility,R2 as 0.630 6and 0.990 2,respectively.Balance time of semen freezing should be between 2hand 3hat 4℃;balance way should adopt the method of water cooling.The experiments showed that further optimizing the key influence factors about semen freezing could improve frozen semen quality of deer.

关 键 词：茸鹿 冻精质量 提高 

分 类 号：S814[农业科学—畜牧学]