猪流行性腹泻病毒LAMP检测方法的建立及初步应用  

Establishment and Preliminary Application of LAMP for Porcine Epidemic Diarrhea Virus Detection

作　　者：时建立[1,2] 彭喆[1,2] 王莉莉 徐胜男[1,2] 张玲玲[2,3] 郑书轩 朱晓琳[1,2] 吴晓燕[1,2] 徐绍建[1,2] 王金宝[1,2] 李俊[1,2] 

机构地区：[1]山东省农业科学院畜牧兽医研究所,山东济南250100 [2]山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [3]青岛农业大学动物科技学院,山东青岛266109

出　　处：《中国动物检疫》2016年第7期82-85,89,共5页China Animal Health Inspection

基　　金：山东省现代农业产业技术体系疫病控制岗位(SDAIT-06-021-07);山东省农业重大应用技术创新课题

摘　　要：猪流行性腹泻病毒是引起仔猪腹泻死亡的主要病原。为实现对猪流行性腹泻病毒(PEDV)的快速检测,利用PEDV S基因设计特异性引物,通过优化各种反应条件,建立了检测PEDV的环介导等温扩增快速检测方法(LAMP)。此方法特异性好,敏感度强,将反转录后的病毒c DNA稀释到108倍仍可检出,是PCR方法的100倍。本研究建立的LAMP方法操作简便、灵敏度高、特异性强,为临床PEDV的快速检测和预防奠定基础。Porcine epidemic diarrhea virus（PEDV）is a major etiological factor of diarrhea and death in piglets. Loop- mediated isothermal amplification（LAMP）assay for rapid detection of PEDV was developed and it could complete de- tection within 60 min. Both agarose gel electrophoresis and naked eyes were able to detect the products in the LAMP as- say. The sensitivity of LAMP could reach to 108 dilutions. These results indicated that the new LAMP method was a very simple and efficient detection method with high sensitivity and specificity for the clinical diagnosis of PEDV in swine farms, which would suit the requirements of rapid detection in laboratory and general conditions.

关 键 词：猪流行性腹泻病毒 环介导等温扩增技术 快速诊断 

分 类 号：S851.3[农业科学—预防兽医学]