缺氧预处理对颅脑损伤大鼠皮层Nrf2及HO-1表达变化的影响  

Expression changes of Nrf2 and HO-1 in rat cortex following traumatic brain injury after hypoxic preconditioning

作　　者：疏龙飞 刘家传[1] 王金标[1] 张永明[1] 张星[1] 杨艳艳[1] 马涛[1] 孙文江[1] 卓健伟 

机构地区：[1]解放军第105医院神经外科,合肥230031

出　　处：《中华神经创伤外科电子杂志》2015年第4期35-39,共5页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

基　　金：全军医学科技"十二五"科研项目(面上)(CWS11J262);2009年度南京军区医学科技创新重点课题(09Z009)

摘　　要：目的研究缺氧预处理对颅脑损伤大鼠皮层核转录因子NF-E2相关因子2(Nrf2)、血红素加氧酶1(HO-1)表达变化的影响。方法 216只SD雄性大鼠,随机分为对照组(Con)、缺氧预处理组(HP)、颅脑损伤组(TBI)、缺氧预处理+颅脑损伤组(HP+TBI);Con和HP组各12只,TBI和HP+TBI组根据伤后处死时间再分为1 h、3 h、6 h、12 h、24 h、3 d、7 d、14 d八亚组,每组12只。采用改良的Feeney's自由落体装置制作颅脑损伤大鼠模型,先在低压氧舱内处理3 d(-50 kPa、3 h/d)制作缺氧预处理模型。四组均用免疫组化和Western blotting测定Nrf2、HO-1的表达变化。结果颅脑损伤后皮层Nrf2和HO-1在伤后1 h开始表达升高,24 h达高峰,3 d后下降,1 h^7 d各时间点,TBI与Con组、HP+TBI与HP组相比,差异有统计学意义(P<0.05)。单纯3 d缺氧预处理后HP组与Con组比较,Nrf2、HO-1的表达差异无统计学意义;伤后1 h^7 d,HP+TBI组Nrf2、HO-1表达较TBI组更高,差异有统计学意义(P<0.05)。结论缺氧预处理进一步增加大鼠颅脑损伤后皮层Nrf2、HO-1表达,提高颅脑损伤后脑组织的抗氧化能力。Objective To investigate the expression changes of nuclear factor erythroid 2-relat-ed factor 2 （Nrf2） and heme oxygenase-1 （HO-1） in rat cortex following traumatic brain injury after hypoxic preconditioning. Methods Two hundred and sixteen adult male Sprague Dawley rats were randomly divided into control group （Con）, hypoxic preconditioning group （HP）, traumatic brain injury group （TBI） and hypoxic preconditioning ＋ traumatic brain injury group （HP ＋TBI）. Con and TBI groups had 12 rats respectively, TBI or HP ＋ TBI group was subdivided into 8 groups according to sacrifice time points, namely group 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, 7 d and 14d （n=12）. TBI models were made by the Feeney’ s improved equipment and hypoxic preconditioning models were made by Hypobaric chamber for 3 days （-50kPa、3 h/d）. The expression of Nrf2 and HO-1 in the brain cortex was detected by immunohistochemistry and western blotting. Results The expression of Nrf2 and HO-1 began to increase at 1 h, reached the peak at 24 h, and decreased slowly at 3 d after TBI. At the time points of 1 h to 7 d, the expression of Nrf2 and HO- 1 in the HP＋TBI or TBI group was significantly higher than those in the Con or HP group （ p〈0.05）. After consecutive three days hypoxic preconditioning, the expression of Nrf2 and HO-1 had no significant difference between HP and Con groups, but the expression of Nrf2 and HO-1 in the HP＋TBI group was higher than those in the TBI group from 1 h to 7 d （p〈0.05）. Conclusion Hypoxic preconditioning up-regulates the expression of Nrf2 and HO-1 in the brain cortex, which enhances the anti-oxidative ability of brain after TBI.

关 键 词：颅脑损伤 核转录因子E2相关因子2 血红素加氧酶1 缺氧预处理 

分 类 号：R651.15[医药卫生—外科学]