针刺对多囊卵巢综合征胰岛素抵抗模型大鼠生殖内分泌及代谢的影响  

Effect of Acupuncture on Reproductive Endocrinology and Metabolism in Polycystic Ovary Syndrome of Insulin Resistance Model Rats

作　　者：林莺[1] 纪峰[1] 黄黎珊[1] 黄云梅[1] 郑良朴[1] 陈曦[1] 杨娟[2] 许金榜[2] 

机构地区：[1]福建中医药大学,福建福州350122 [2]福建省妇幼保健院,福建福州350001

出　　处：《广西中医药大学学报》2016年第2期1-5,共5页Journal of Guangxi University Of Chinese Medicine

基　　金：国家自然科学基金(编号:81302998);福建省科技计划项目(编号:2015Y0002);福建省中医药科研项目(编号:wzfg201304);福建省卫生计生委青年科研课题(编号:2014-1-20)

摘　　要：[目的]观察针刺对多囊卵巢综合征胰岛素抵抗(PCOS-IR)模型大鼠生殖内分泌及代谢的影响。[方法]采用来曲唑灌胃联合高脂膳食建立PCOS-IR模型,模型大鼠随机分为模型对照组、针刺组、二甲双胍组,每组6只;另设6只同期喂养的15周龄雌性SD大鼠作为空白对照组。针刺组予针刺穴位(一组为关元、三阴交,另一组为肝俞、脾俞、肾俞穴)治疗,每天1次,两组穴位轮替使用,连续治疗20 d;二甲双胍组给予二甲双胍溶液灌胃;模型对照组给予与针刺组相同的抓取、固定、穴位剪毛、消毒,不做其他任何治疗;空白对照组自由摄食、饮水。各组大鼠治疗后检测血清性激素,观察体重、动情周期和卵巢组织学变化,测定血脂水平、糖耐量、空腹血糖(FPG)和空腹胰岛素(FINS)浓度,并计算胰岛素抵抗指数(HOMA-IR)。[结果]1模型对照组大鼠卵巢呈多囊样改变,卵巢闭锁卵泡增多,闭锁卵泡的直径较大,颗粒细胞层数有所减少,未见黄体分布;针刺组和二甲双胍组卵巢恢复排卵,切片可见新鲜黄体分布。2与模型对照组比较,针刺组血清T、LH、PRL水平明显降低,FSH、E2、P水平明显升高(P<0.05或P<0.01)。3与模型对照组比较,针刺组及二甲双胍组治疗后大鼠体重增加速度明显减慢(P<0.01),肥胖指数(Lee’s指数)明显降低(P<0.01);血清FINS含量及HOMA-IR显著降低(P<0.01),糖耐量曲线下面积明显减少(P<0.05或P<0.01);血清总胆固醇水平显著降低(P<0.01),血清低密度脂蛋白显著降低(P<0.01),针刺组与二甲双胍治疗组差异无统计学意义。[结论]针刺能够改善PCOS-IR模型大鼠生殖内分泌异常和胰岛素抵抗。[Objective]To observe the effect of acupuncture on reproductive endocrinology and metabolism in Polycystic Ovary Syndrome of Insulin Resistance（PCOS-IR）rats.[Methods]PCOS-IR model rats were established with Letrozole lavage and high-fat diet. Model rats were divided randomly into the model control group,the acupuncture group and the metformin group（6 rats for each group）. The other six female SD rats（15 weeks old）fed at the same time were included as the blank group. The rats of the acupuncture group were treated with acupuncture every day at the following points for 20 days： Guanyuan（CV4）,Sanyinjiao（SP6） for one group;Shenshu（BL23）,Pishu（BL20）,Ganshu（BL18） for another. The two groups of points were applied alternately. The rats of the metformin group was intragastrically administrated with metformin solution. The rats of the model control group were given the same crawl,fixed points shearing,disinfection of acupuncture group without any other treatment. Blank control group had free access to food and drinking water. Serum levels of sex hormones were detected after treatment for each group. The changes of weight,estrous cycle and ovarian morphology were observed. The lipid levels,glucose tolerance,fasting plasma glucose（FPG） and fasting insulin（FINS）concentration was determined. And insulin resistance index（HOMA-IR） was calculated.[Results]1Model group rat ovary showed multiple cystic changes,an increase in ovarian follicular atresia with larger diameter particles,decreased layers of granulosa cells,and no corpora lutea distribution. Acupuncture group and metformin group restore varies ovulation. The distribution of fresh corpus luteum was shown in section. 2 Compared with model group,serum T,LH,PRL levels of acupuncture group were significantly decreased,and FSH,E2,P levels were significantly increased（P0.05 or P0.01）. 3Compared with model group,weight gain of rats in acupuncture group and metformin group has slowed significantly（P0.01）. Obesity index（Lee�

关 键 词：针刺 多囊卵巢综合征 胰岛素抵抗 生殖内分泌 糖脂代谢 

分 类 号：R711.75[医药卫生—妇产科学]