琥乙红霉素纳米乳的制备及其体外抑菌活性研究  

Preparation of Erythromycin Nanoemulsion and Determination of Its Bacteriostatic Activity in vitro

作　　者：郑星星 欧阳五庆[2] 

机构地区：[1]榆林农业学校,陕西榆林719000 [2]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《安徽农业科学》2016年第13期183-185,共3页Journal of Anhui Agricultural Sciences

基　　金：陕西省重大科技创新专项(K332020916)

摘　　要：[目的]制备琥乙红霉素纳米乳,并对其进行体外抑菌活性研究。[方法]利用伪三元相图法筛选琥乙红霉素纳米乳处方;采用微量肉汤稀释法,检测琥乙红霉素纳米乳对致病性大肠埃希菌、金黄色葡萄球菌、肺炎球菌和变形杆菌的最小抑菌浓度(MIC)和最小杀菌质量浓度(MBC)。[结果]琥乙红霉素纳米乳中各组分的质量分数如下:琥乙红霉素2.8%、乳酸甲氧苄啶0.6%、EL-40 35.2%、肉桂醛7.5%、蒸馏水53.9%。体外抑菌试验表明,琥乙红霉素纳米乳对致病性大肠埃希菌、金黄色葡萄球菌、肺炎球菌和变形杆菌的最小抑菌浓度(MIC)分别为4、1、2和4μg/m L,其抑菌效果明显优于其他对照药物组。[结论]该研究成功研制了琥乙红霉素纳米乳,其体外抑菌效果明显。[Objective] To prepare erythromycin nanoemulsion,and to research its bacteriostatic activity in vitro. [Method] The preparation of the erythromycin nanoemulsion was optimized by studying the pseudoternary phase diagram. Broth dilution method was used to determine the MIC and MBC of erythromycin nanoemulsion on enteropathogenic Escherichia coli,Staphylococcus aureus,pneumococcus and proteus. [Result]The mass fraction of the components in erythromycin nanoemulsion included 2. 8% rythromycin,0. 6% trimethoprim lactate,35. 2% EL-40,7. 5% cinnamaldehyde,and 53. 9% distilled water. The susceptibility test revealed that the MIC of erythromycin nanoemulsion on enteropathogenic E. coli,S. aureus,pneumococcus and proteus was 4,1,2 and 4 μg / m L,respectively. Their antibacterial effects significantly was better than other drugs. [Conclusion]The erythromycin nanoemulsion is successfully prepared,and its antibacterial effect in vitro is remarkable.

关 键 词：头孢克肟 纳米乳 伪三元相图 最小抑菌浓度 

分 类 号：S859.53[农业科学—临床兽医学]