拟南芥热激因子AtHsfA1a对细胞程序性死亡形态的影响  

Effect of Arabidopsis Heat Shock Factor AtHsfA1a on Morphology of Programmed Cell Death

作　　者：曾洁媛 徐娅[1] 李念[1] 郤秋霞 郭丽红[1] 

机构地区：[1]昆明学院云南省高校特色生物资源开发与利用重点实验室,云南昆明650214

出　　处：《昆明学院学报》2016年第3期70-73,共4页Journal of Kunming University

基　　金：国家自然科学基金资助项目(31260061;31060039);校级课题资助项目(YJL11025);校级重点学科建设资助项目;大学生创新资助项目

摘　　要：实验以热激因子At Hsf A1a不同基因型(野生型W和沉默型N)的拟南芥为材料,诱导其形成愈伤组织,将愈伤组织进行悬浮培养至单细胞,热激处理后,经过DAPI染色,在荧光显微镜下观察细胞涂片.结果表明,野生型拟南芥的单细胞均呈大小一致的完整圆形,且染色均匀,为正常的细胞形态.基因沉默型局部发生细胞质的浓缩,出现凋亡小体,说明拟南芥热激因子At Hsf A1a对细胞程序性死亡有一定的抑制作用.In the test,Arabidopsis heat shock factor At Hsf Ala with different gene types（ wild W and mute N） was used to induce forming the healing tissue. Single cell was then gained by suspension culture from the tissue. After heat shock treatment,the single cell was treated with DAPI staining,and observed by fluorescence microscope. The results show that the morphology of single cell of the wild type is normal with the full circle in same size,and uniform staining. In At Hsf A1 a silencing type the cytoplasm concentrated in some part appearing with apoptotic body. These show that Arabidopsis heat shock factor At Hsf Ala has certain inbibitional effect on programmed cell death.

关 键 词：拟南芥 热激因子AtHsfA1a 细胞程序性死亡 显微观察 

分 类 号：Q943.2[生物学—植物学]