先天性心脏病合并肺动脉高压患儿血清Rho激酶、内皮素1和一氧化氮表达的意义  

Significance of expressions of serum Rho kinase,endothelin-1 and nitric oxide in children with congenital heart disease and pulmonary hypertension

作　　者：李加新[1] 孙兴珍[1] 程学英[2] 姜玉章[1] 谭维琴[1] 

机构地区：[1]南京医科大学附属淮安第一医院儿科,江苏省223300 [2]南京市儿童医院心脏病科

出　　处：《江苏医药》2016年第12期1348-1350,共3页Jiangsu Medical Journal

基　　金：江苏省"333工程"培养资金资助项目(BRA2015151)

摘　　要：目的探讨Rho激酶、内皮素1(ET-1)和一氧化氮(NO)表达在先天性心脏病(CHD)合并肺动脉高压发病机制中的意义。方法将90例CHD患儿分为无肺动脉高压组(A组,30例)、轻度肺动脉高压组(B组,30例)和中、重度肺动脉高压组(C组,30例);另选择同期健康体检儿30例为对照组(D组)。采用ELISA法、放射免疫法和硝酸还原酶法分别检测血清Rho激酶、ET-1和NO水平,并分析其与肺动脉收缩压(PASP)的相关性。结果与D组相比,A、B、C组血清Rho激酶和ET-1水平升高,而NO水平降低(P<0.01)。B、C组血清Rho激酶和ET-1水平高于A组,而NO水平低于A组(P<0.01)。PASP与血清Rho激酶、ET-1水平呈正相关(r=0.707、0.888,P<0.01),而与NO水平呈负相关(r=-0.696,P<0.01)。血清Rho激酶、ET-1与NO水平呈负相关(r=-0.522、-0.592,P<0.01)。结论 CHD患儿血清Rho激酶、ET-1、NO水平的变化在肺动脉高压发生和发展中起重要作用。Objective To investigate the significance of expressions of Rho kinase,endothelin-1 （ET-1） and nitric oxide（NO） in the pathogenesis of congenital heart disease（CHD） associated with pulmonary hypertension. Methods A total of 90 children with CHD was divided into three groups of A（CHD without pulmonary hypertension, 30 cases）, B（CHD with mild pulmonary hypertension, 30 cases） and C（CHD with moderate and severe pulmonary hypertension, 30 cases）. Another 30 healthy children were taken as the controls（group D）. Serum levels of Rho kinase, ET-1 and NO were detected by ELISA, radioimmunoassay and nitrate reductase method, respectively. The correlation between the expressions and pulmonary artery systolic pressure （PASP） was analyzed. Results Compared with group D, serum levels of Rho kinase and ET-1 were increased, while those of NO were decreased in groups of A, B and C（P〈0. 01）. Serum levels of Rho kinase and ET-1 were higher, but NO was lower,in groups of B and C than those in group A（P〈0.01）. PASP was positively correlated with Rho kinase and ET-1 （r=0. 707 and r=0. 888, P〈0.01）, but negatively correlated with NO （r=-0. 696,P〈0. 01）. There was a negative correlation between the expressions of Rho kinase, ET-1 and NO（r=-0. 522 and r=-0. 592,P〈0. 01）. Conclusion Serum levels of Rho kinase,ET-1 and NO in children with CHD may play an important role in the development and progression of pulmonary hypertension.

关 键 词：先天性心脏病 肺动脉高压 RHO激酶 内皮素1 一氧化氮 

分 类 号：R725[医药卫生—儿科]