鱼精蛋白包覆PLA纳米囊对小鼠骨髓系树突状细胞免疫功能的影响  

Effect of Protamine Coated PLA Nanocapsule on the Immune Function of Murine BMDCs In Vitro

作　　者：杨萍[1,2] 朱俊铭[1,2] 

机构地区：[1]武汉生物技术研究院,武汉430075 [2]华中科技大学生命科学与技术学院,武汉430074

出　　处：《华中科技大学学报（医学版）》2016年第3期298-301,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：武汉市关键技术攻关计划资助项目(No.2014061305090453)

摘　　要：目的探索鱼精蛋白(PS)包覆聚乳酸(PLA)纳米囊对小鼠骨髓系树突状细胞(BMDC)体外免疫应答效应的影响。方法采用复乳溶剂挥发法制备包裹模型抗原鸡卵清白蛋白(OVA)的PLA纳米囊,以PS进行表面修饰。将纳米囊作用于体外培养的BMDC,用流式细胞仪检测BMDC表面分子CD80、CD83、CD86、MHCⅠ、MHCⅡ的表达和对纳米囊的摄取情况,用ELISA法检测BMDC分泌IL-12p70水平。结果 PS包覆PLA纳米囊可显著促进BMDC表达CD80、CD83、MHCⅠ和MHCⅡ表面分子,增加BMDC分泌IL-12p70,增强BMDC对OVA的摄取。结论PS包覆PLA纳米囊可以增强BMDC的免疫应答效果,有望成为一种良好的药物载体。Objective To investigate the effect of protamine（PS）coated polylactic acid（PLA）nanocapsule on the immune response of murine bone marrow-derived dendritic cells（BMDCs）.Methods PLA nanocapsules were prepared by W1/O/W2 method.Protamine was used to modify the surface of PLA nanocapsule that encapsulated ovalbumin（OVA）,a model anti-gen.The prepared OVA/PLA/PS nanocapsules were used to stimulate BMDCs.Flow cytometry was used to analyze the ex-pression of surface molecules CD80,CD83,CD86,MHCⅠ,and MHC Ⅱ,and the uptake of nanocapsule.The levels of IL-12 p70 secreted by BMDCs were detected by ELISA.Results OVA/PLA/PS nanocapsules could significantly up-regulate the expres-sion of CD80,CD83,MHCⅠ,and MHC Ⅱ in BMDCs,and increase the secretion of IL-12 p70 by BMDCs.Furthermore,OVA/PLA/PS nanocapsules could enhance the uptake efficiency of OVA by BMDCs.Conclusion OVA/PLA/PS nanocapsule can en-hance the immune response of BMDCs,and may become a good drug delivery carrier.

关 键 词：聚乳酸 鱼精蛋白 纳米囊 树突状细胞 免疫功能 

分 类 号：R392.12[医药卫生—免疫学]