瑞芬太尼诱发大鼠切口痛觉过敏时脊髓CXCL10及其配体CXCR3的表达变化及意义  

Changes of the Expression of CXCL10 and Its Ligand CXCR3 in Spinal Cord during Remifentanil-induced Hyperalgesia in Rats with Incisional Pain

作　　者：雷鹏飞[1] 高杉[1] 薛然荣[1] 王慧玲[1] 

机构地区：[1]河南省新乡市中心医院麻醉科,新乡453000

出　　处：《华中科技大学学报（医学版）》2016年第3期306-310,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨瑞芬太尼诱发大鼠切口痛觉过敏时脊髓CXC类趋化因子10（CXCL10）及其配体CXC趋化因子受体3（CXCR3）表达变化及意义。方法 30只健康雌性SD大鼠随机分为假手术组、切口痛模型组、瑞芬太尼＋切口痛模型组,每组10只。制备切口痛模型,瑞芬太尼＋切口痛模型组以1μg/（kg·min）速率从尾静脉输注瑞芬太尼60min,假手术组和切口痛组以同样的速率输注生理盐水。分别于建模前12h（T_0）、输注瑞芬太尼4h（T_1）、12h（T_2）、24h（T_3）和48h（T_4）,测定大鼠机械缩足反应阈（PWT）和热缩足潜伏期（TWL）,于T_4时测定完PWT和TWL后,处死动物并取骨髓,利用实时荧光定量PCR测定大鼠脊髓组织中CXCL10和CXCR3基因表达,Western blot法检测大鼠脊髓组织中CXCL10和CXCR3蛋白表达。免疫组织荧光染色检测脊髓组织中小胶质细胞标志物Iba1的表达。结果与T_0时相比,切口痛模型组和瑞芬太尼＋切口痛模型组大鼠T_（1~4）时PWT和TWL均降低（均P〈0.05）;瑞芬太尼＋切口痛模型组大鼠T_（1~4）时PWT和TWL均低于切口痛模型组和假手术组,差异均有统计学意义（均P〈0.05）;与假手术组相比,切口痛模型组和瑞芬太尼＋切口痛模型组大鼠脊髓组织中CXCL10和CXCR3基因和蛋白表达量均升高,差异均有统计学意义（均P〈0.05）,与切口痛模型组相比,瑞芬太尼＋切口痛模型组大鼠脊髓组织中CXCL10和CXCR3基因和蛋白表达量均升高,差异均有统计学意义（均P〈0.05）。免疫荧光染色结果显示,与假手术组和切口痛模型组相比,瑞芬太尼＋切口痛模型组Ibal表达量显著增加,小胶质细胞体积增大,分支增多。结论瑞芬太尼诱发大鼠切口痛觉过敏时脊髓中CXCL10及其配体CXCR3表达水平升高,可能与CXCL10作用于神经元表面CXCR3而间接诱导小胶质细胞活化有关。Objective To investigate the changes of the expression of CXC chemokine 10（CXCL10）and its ligand CXC che-mokine receptor 3（CXCR3）in spinal cord during remifentanil-induced hyperalgesia in rats with incisional pain.Methods Thirty healthy female SD rats were randomly divided into sham group,incisional pain model group,remifentanil＋incisional pain model group,each group having 10 rats.Incisional pain models were established.In remifentanil＋incisional pain model group,remifen-tanil was infused at 1μg/（kg＆#183;min）for 60 min via the tail vein,and in sham group and incision pain model group,normal saline was given in the same manner.The mechanical paw withdrawal threshold（PWT）and thermal withdrawal latency（TWL）were measured 12 h before modeling（T0 ）,4 h（T1 ）,12 h（T2 ）,24 h（T3 ）and 48 h（T4 ）after infusion of remifentanil.The expression of CXCL10 and CXCR3 in rat spinal cord tissues was detected at mRNA level by real-time quantitative PCR assay and at protein level by Western blotting at T4 after detection of the PWT and TWL.Results The PWT and TWL of rats were significantly decreased at T1-4 when compared with T0 in incisional pain model group and remifentanil＋incisional pain model group（P〈0.05）,and they were lower in remifentanil＋incisional pain model group than in the incisional pain model group and the sham group at T1-4 ,with the difference being statistically significant（P〈0.05）.Compared with the sham group,the expressions lev-els of CXCL10 and CXCR3 mRNA and protein in the rat spinal cord tissues in incisional pain model group and remifentanil＋in-cisional pain model group were increased,and the differences were statistically significant（P〈0.05）.The expressions levels of CXCL10 and CXCR3 mRNA and protein were markedly increased in the rat spinal cord tissues in remifentanil＋incisional pain model group relative to the incisional pain model group（P〈0.05）.Immunofluorescence staining showed that the expression levels of Ibal,a marker of micr

关 键 词：瑞芬太尼 痛觉过敏 CXC类趋化因子10 CXC趋化因子受体3 

分 类 号：R614.2[医药卫生—麻醉学]