肺结核治疗过程中血清IL-10、IL-12p40、IL-12p70、IL-17A、IL-4、IL-6和IP10水平动态观察  

Dynamic Survey of IL- 10,IL- 12p40,IL- 12p70,IL- 17A,IL- 4,IL- 6 and IP10 in Serum during Treatment of Patients with Pulmonary Tuberculosis

作　　者：劳穗华[1] 谢贝[1] 董海平[1] 张言斌[1] 孟繁荣[1] 俞朝贤[1] 陈华 邓晓华[1] 王楠[1] 杨瑜[1] 雷杰[1] 牛群[1] 刘志辉[1] 

机构地区：[1]广州市胸科医院,广东广州510095

出　　处：《现代医院》2016年第8期1172-1174,共3页Modern Hospitals

基　　金：广东省科技计划项目(编号:粤科规财字[2015]110号32);广东省医学科学技术研究基金(编号:A2016383);广州市科技计划项目(编号:2014Y2-00117;155700012)

摘　　要：目的分析肺结核患者治疗过程中血清IL-10、IL-12p40、IL-12p70、IL-17A、IL-4、IL-6和IP10水平的动态变化,以进一步阐明这些因子与结核病发生发展的关联性。方法应用液相芯片技术检测59例成功治愈的初治菌阳肺结核患者治疗前、治疗2个月末和6个月末的血清IL-10、IL-12p40、IL-12p70、IL-17A、IL-4、IL-6和IP10水平,应用配对t检验分别比较各因子3个时点间的血清表达水平差异。结果 3个时间点血清IL-10、IL-12p40、IL-12p70、IL-17A、IL-4、IL-6和IP10水平(pg/m L):治疗前分别为1.71±0.91、5.02±11.53、2.48±11.03、1.12±0.46、0.36±1.87、8.99±7.82和882.74±1 110.73,治疗2个月末分别为2.39±1.47、8.95±13.76、1.77±1.80、0.96±0.22、0.51±1.11、4.75±4.16和408.38±492.27,治疗6个月末分别为1.84±0.93、5.14±10.16、1.59±0.78、0.79±0.22、0.44±0.48、4.05±4.51和177.57±104.83。治疗前与治疗2个月末及治疗6个月末的IL-6水平、治疗前与治疗2月末的IL-10和IL-12p40水平以及治疗前、治疗2个月末、6个月末的IL-17A和IP10水平均有显著性差异(P<0.05)。结论血清IL-17A、IP10、IL-6、IL-10和IL-12p40水平与结核病的发生发展存在密切关联。Objective To further understand the correlation between progression of tuberculosis with these cytokines by dynamic analysis of levels of IL- 10,IL- 12p40,IL- 12p70,IL- 17 A,IL- 4,IL- 6 and IP10 in serum during treatment of patients with pulmonary tuberculosis. Methods IL- 10,IL- 12p40,IL- 12p70,IL- 17 A,IL- 4,IL- 6 and IP10 in serum of 59 patients with pulmonary tuberculosis and treated successfully at three point of time,including before treatment and the end of second and sixth month during period of treatment,were detected with liquid chip technology. The general levels of these cytokines at per point of time were compared respectively by paired t-test. Results The general levels（ pg/ml） of IL- 10,IL- 12p40,IL- 12p70,IL- 17 A,IL- 4,IL- 6 and IP10 in serum were 2. 39 ± 1. 47,8. 95 ± 13. 76,1. 77 ± 1. 80,0. 96 ± 0. 22,0. 51 ± 1. 11,4. 75 ± 4. 16,408. 38 ± 492. 27 at the end of second month and 1. 84 ± 0. 93,5. 14 ± 10. 16,1. 59 ± 0. 78,0. 79 ± 0. 22,0. 44 ± 0. 48,4. 05 ± 4. 51,177. 57 ± 104. 83 at the end of sixth month during period of treatment while they were 1. 71 ± 0. 91,5. 02 ± 11. 53,2. 48 ± 11. 03,1. 12 ± 0. 46,0. 36 ± 1. 87,8. 99 ± 7. 82 and882. 74 ± 1 110. 73 before treatment respectively. There were significantly differences between the levels of IL- 6 at the end of second month and that before treatment and between that at sixth month and that before treatment. There were also significantly differences between the levels of IL- 10 and IL- 12p40 at the end of second month and that before treatment. As to IL- 17 A and IP10,there were significantly differences between each other of their levels at three point of time. Conclusion It seems probable that there are strong correlations between progression of tuberculosis and levels of IL- 17 A,IP10,IL- 6,IL- 10 and IL- 12p40 in serum.

关 键 词：结核/肺 细胞因子 液相芯片 

分 类 号：R521[医药卫生—内科学] R446.6[医药卫生—临床医学]