硅胶—硝酸银硅胶柱层析分离纯化β-倍半水芹烯  

Separation of β-sesquiphellandrene by silica gel and argentation column chromatography

作　　者：王艳[1] 周长远 杜爱玲[3] 

机构地区：[1]山东职业学院生物工程系,山东济南250104 [2]枣庄市台儿庄区林业局,山东枣庄277400 [3]山东大学化学与化工学院,山东济南250061

出　　处：《食品与机械》2016年第5期165-167,228,共4页Food and Machinery

基　　金：科技部农业科技成果转化项目(编号:2008GB2C600178)

摘　　要：利用普通硅胶柱,选用乙醚—正己烷作流动相对姜油树脂进行初步分离,采用GC—MS法进行跟踪检测。对比不同展开剂下β-倍半水芹烯和姜烯混合液在硝酸银硅胶板上的层析情况,选用体积比为991的正己烷—乙酸乙酯流动相体系分离其中的β-倍半水芹烯。结果表明:β-倍半水芹烯的GC—MS峰面积百分数达95.79%,收率为89.73%。通过对β-倍半水芹烯峰值样进行HPLC检测,证明β-倍半水芹烯的紫外特征吸收波长为232nm,在姜烯的特征吸收峰262nm下基本无吸收。A aether and n-hexane solution was used as mobile phase to preliminary extractβ-sesquiphellandrene from ginger oleoresin by common silica gel column chromatography,and GC—MS was used for detectingβ-sesquiphellandrene tracing.Comparison of different developing solvents ofβ-sesquiphellandrene and zingiberene on argentated silica gel G pre-coated plate,hexane-ethyl acetate（991）was used as the mobile phase to sepatateβ-sesquiphellandrene,the results indicated that the GC—MS area percentage of the silica gel and argentation column chromatography product was 95.79% with 89.73% recovery.Through the HPLC detection ofβ-sesquiphellandrene peak sample,β-sesquiphellandrene was found its existence of UV absorption at 232 nm,and generally had no absorption at 262 nm the characteristic absorption of zingiberene.

关 键 词：硅胶 硝酸银硅胶 β-倍半水芹烯 柱层析 

分 类 号：O652.6[理学—分析化学] O629[理学—化学]