肌醇六磷酸或(和)肌醇对多种癌细胞增殖影响  

EFFECT OF INOSITOL HEXAPHOSPHATE AND/OR MYO-INOSITOL ON PROLIFERATION OF MULTIPLE CANCER CELLS

作　　者：王鹏[1] 宋扬[1] 高丽鹤 付敏[1] 陈沉[1] 

机构地区：[1]青岛大学医学院营养研究所,山东青岛266021

出　　处：《齐鲁医学杂志》2016年第3期301-303,306,共4页Medical Journal of Qilu

摘　　要：目的观察肌醇六磷酸（IP6）、肌醇（Ins）及两者合用对多种癌细胞增殖的抑制效果。方法取培养至对数生长期的人肝癌细胞Hep G2、人结肠癌细胞HT-29、人胃癌细胞7901、人乳癌细胞MCF-7和人肺癌细胞A549,接种于96孔板中,分别加入0.5、1.0、2.0、4.0 mmol/L的IP6、Ins及IP6＋Ins（1∶1,V/V）混合液,另设对照组（不加药物）。应用四甲基偶氮噻唑蓝（MTT）法测定不同浓度的IP6、Ins和两种药物合用作用于不同癌细胞48 h的抑制率,计算IP6、Ins及两种药物合用对不同癌细胞的半数抑制浓度（IC50）。结果与对照组比较,不同浓度的IP6、Ins及两种药物合用对5种癌细胞均能产生显著的抑制作用（F=8.253~15.292,t=6.871~8.537,P〈0.05）。不同浓度的IP6、Ins及两种药物合用对HT-29、7901、MCF-7、A549细胞抑制率差异无显著性（P〉0.05）;但在4.0mmol/L时,合用组对Hep G2细胞抑制效果显著高于IP6组和Ins组（F=7.462,P〈0.05）。IP6、Ins及两种药物合用作用于不同癌细胞的IC50比较,差异有显著性（F=6.792~17.694,P〈0.05）,其中Hep G2细胞和HT-29细胞的IC50值较低。结论 IP6、Ins及两种药物合用对不同癌细胞抑制效果不同,两种药物合用对5种癌细胞增殖抑制的增强作用不明显。Objective To observe the inhibitory effect of IP6,inositol and a combination of the two on proliferation of multiple cancer cells. Methods The human liver cancer cell Hep G2,colon cancer cell HT-29,gastric cancer cell 7901,breast cancer cell MCF-7 and lung cancer cell A549 in logarithmic growth phase were collected and vaccinated in 96 well plates,and 0. 5,1. 0,2. 0 and 4. 0 mmol /L of IP6,inositol and IP6 ＋ inositol were added in,respectively,a control group was found with no medication added. The 48 h inhibition action of different concentration of IP6,inositol and a combination of IP6 and inositol on different cancer cells was detected using MTT assay,and half inhibitory concentration（ IC50） of IP6,inositol and a combination of the two were calculated. Results The results showed that different concentrations of IP6,inositol and a combination of the two had a significant inhibition effect on the five kinds of cancer cells（ F =8. 253- 15. 292,t = 6. 871- 8. 537,P 〈 0. 05）. The differences in inhibition action of different concentrations of IP6,inositol and a combination of the two on HT-29,7901,MCF-7 and A549 cells were not significant（ P 〉 0. 05）,but when the concentration was 4. 0 mmol / L,the inhibition action of IP6 ＋ inositol on Hep G2 cells was stronger than that in IP6 and inositol（ F = 7. 462,P 〈 0. 05）. The differences in the effect of IP6,inositol and a combination of the two on IC50 of different cancer cells were significant（ F = 6. 792- 17. 694,P 〈 0. 05）,of which,the IC50 of Hpe G2 and HT-29 was much lower than the others. Conclusion The inhibitory effects of IP6 and / or inositol on the five kinds of cancer cells are different,and the enhancement of cell growth inhibition of a combination of the two drugs on the five kinds of cancer cells is not so obvious.

关 键 词：植酸 肌醇 细胞系 肿瘤 细胞增殖 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]