γ干扰素处理对树突状细胞生物学特性及功能的影响  

Effects of IFN-γ treatment on biological characteristics and functions of dendritic cells

作　　者：刘燕玲[1,2] 王红梅[1] 余艳荣 袁铿 张瑜娟 闵卫平[1,3] 

机构地区：[1]南昌大学免疫与生物治疗研究所,江西南昌330006 [2]江西科技学院,江西南昌330006 [3]江西省医学科学院,江西南昌330006

出　　处：《细胞与分子免疫学杂志》2016年第8期1009-1013,1020,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81273303);江西省重大科技创新项目(20124ACB00800);江西省重大科技计划重点项目(2013BBG70019);江西省研究生创新专项基金(YC2013-B006)

摘　　要：目的研究γ干扰素(IFN-γ)处理对C57BL/6小鼠树突状细胞(DC)生物学特性及功能的影响。方法在DC培养过程的早期(第2天)或晚期(第5天)加入20 ng/m L IFN-γ进行处理,第7天加入脂多糖(LPS)刺激DC成熟,流式细胞术检测细胞表面分子CD11c、CD80、CD86的表达。为检测DC功能,将其与BALB/c小鼠来源的淋巴细胞进行共培养,5,6-羧基荧光黄二乙酸盐N-琥珀酰亚胺酯(CFSE)标记法检测其刺激异基因淋巴细胞增殖的能力、流式细胞术检测其诱导调节性T细胞(Treg)产生的能力。结果与对照组相比,早期IFN-γ处理组的DC数量减少、分化受到抑制;虽然表面共刺激分子的表达无明显变化,但是却明显拮抗LPS刺激其成熟;刺激异基因淋巴细胞增殖的能力明显减弱;诱导产生更多的Treg。与对照组相比,晚期IFN-γ处理的DC数量及分化无变化;表面共刺激分子CD86及CD80表达升高;LPS刺激DC成熟及同种异体混合淋巴细胞反应的结果相似,但是诱导Treg产生的能力增加。结论早期IFN-γ处理组和晚期IFN-γ处理组的DC生物学特性及功能明显不同。Objective To investigate the effect of IFN-y treatment on the biological characteristics and functions of C57BL/6 murine dendritic cells （DCs）. Methods In the process of DC culture, 20 ng/mL IFN-y was added in the DCs at the early （day 2） or late （day 5） stage, and on day 7, LPS was added to stimulate DC maturation. The expressions of DC surface molecules CDllc, CDS0 and CD86 were determined by flow cytometry. To analyze cell functions, DCs were co-cultured with BALB/c mouse-derived lymphocyte cells. The 5, 6-carboxyfluorescein diacetate N-succinimidyl ester （CFSE） labelling was used to detect their ability to stimulate allogeneic lymphocyte proliferation and flow cytometry was used to measure their ability to induce the production of regulatory T cells （Tregs）. Results Compared with the control group, the early IFN-y treatment group had decreased DC number and inhibited cell differentiation; though there was no difference in the expression of co-stimulatory molecules, early IFN-y treatment resisted the stimulatory effect of LPS on DC maturation, weakened the ability to stimulate allogeneic lymphocyte proliferation and enhanced the ability to induce more Tregs. Compared with the control group, the late IFN-γ treatment group showed no change in DC number and differentiation; the expressions of co-stimulatory molecules CD86 and CD80 were upregulated; the results of DC maturation and mixed allogeneic lymphocyte reaction stimulated by LPS were similar to those in the control group, but its ability to induce Tregs was stronger. Conclusion DCs treated with IFN-γ, at early stage and those at late stage showed obvious difference in biological characteristics and functions.

关 键 词：树突状细胞 1干扰素(IFN-γ) 混合淋巴细胞反应 脂多糖 调节性T细胞 

分 类 号：R392.11[医药卫生—免疫学]