生长相关蛋白43(GAP43)基因修饰的骨髓间充质干细胞移植对大鼠视网膜变性疾病的治疗作用  

Therapeutical effect of growth-associated protein 43(GAP43) gene-modified bone marrow mesenchymal stem cell transplantation on rat retinal degenerative diseases

作　　者：杨娟[1] 谢茂松[1] 郑卫东[1] 胡建章[1] 曲强[1] 

机构地区：[1]福建医科大学附属第一医院眼科,福建福州350004

出　　处：《细胞与分子免疫学杂志》2016年第8期1041-1044,1050,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：福建省卫计委中青年骨干人才培养资助项目(2014-ZQN-ZD-16)

摘　　要：目的探讨生长相关蛋白43(GAP43)基因修饰的骨髓间充质干细胞(BMSC)移植对治疗视网膜变性(RP)疾病的前景。方法采用贴壁法进行分离培养BMSC,经慢病毒载体将GAP43基因导入BMSC得到GAP43基因修饰的BMSC。将63只皇家外科学院(RCS)大鼠按随机数字表随机分为3组:实验组、阴性对照组和空白对照组。实验组接受视网膜下注射GAP43基因修饰的BMSC混悬液,阴性对照组组接受视网膜下注射BMSC混悬液,空白对照组接受视网膜下注射PBS。移植后30 d,通过光学相干断层扫描(OCT)检测视网膜厚度,通过Western blot法检测视紫红质在RCS大鼠视网膜中的表达。结果与空白对照组和阴性对照组相比,GAP43基因修饰的BMSC移植30 d后,实验组视网膜厚度显著增加,视网膜视紫红质明显增强。结论 GAP43基因修饰的BMSC移植能增加光感受细胞的存活数,延缓视网膜变性。Objective To investigate the potential of the treatment of growth-associated protein 43 （GAP43） gene-modified bone marrow-derived mesenchymal stem cells （BMSCs） for retinitis pigmentosa （RP）. Methods BMSCs were isolated and cultured by adherence method. By transfecting GAP43 gene into BMSCs via a lentivirus vector, we got GAP43 gene-modified BMSCs. Sixty-three Royal College of Surgeons （RCS） rats were randomly divided into three groups： experimental group, negative control group and blank control group. The experimental rats received subretinal injection of GAP43 gene-modified BMSCs. The negative control rats received subretinal injection of BMSCs. The control rats received subretinal injection of PBS. Thirty days after transplanting, the retinal thickness was detected by optical coherence tomography （OCT）, and the expression of rhodopsin in RCS rat retinas was examined by Western blotting. Results Compared with the blank control group and the negative control group, 30 days after GAP43 gene-modified BMSC transplantation, the retinal thickness of the experimental group remarkably increased and the expression of rhodopsin significantly rose. Conclusion GAP43 gene-modified BMSC transplantation can increase survival photoreceptor cells and delay retinal degeneration.

关 键 词：视网膜变性疾病 骨髓间充质干细胞 基因修饰 

分 类 号：Q78[生物学—分子生物学]