参芪扶正注射液促进环磷酰胺处理小鼠肠道相关淋巴组织B细胞数量的恢复  

Shengqifuzheng Injection promotes the recovery of B cells in gut-associated lymphoid tissues of mice treated with cyclophosphamide

作　　者：邓向亮[1,2] 黄容容[1] 温如燕[1] 罗霞[1] 周联[1] 

机构地区：[1]广州中医药大学中药学院 [2]无限极中草药免疫研究中心,广东广州510006

出　　处：《细胞与分子免疫学杂志》2016年第8期1073-1077,1082,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：高等学校博士学科点专项科研基金(20124425110010);广州中医药大学中药学院本科生科研培育项目(2014年)

摘　　要：目的考察参芪扶正注射液(SQFZ)对环磷酰胺(Cy)处理后小鼠肠道相关淋巴组织(GALT)B细胞数量恢复的影响。方法将BALB/c小鼠随机分为正常对照组、Cy组和SQFZ组。Cy组和SQFZ组小鼠均腹腔注射Cy(100 mg/kg),对照组给予等量生理盐水;24 h后,SQFZ组小鼠灌服SQFZ 1 m L/只,其余2组给予等量的生理盐水,每天灌胃1次连续10 d;每天记录小鼠体质量。给药第10天处死小鼠,测定胸腺指数和脾指数;流式细胞术检测肠系膜淋巴结(MLN)和Peyer集合淋巴结(PP)淋巴细胞中B细胞比例及股骨骨髓细胞周期。体外实验中,小鼠淋巴细胞经SQFZ处理后,MTT法检测细胞活性,流式细胞术检测B细胞表达CD86分子水平;羧基荧光素二乙酸盐琥珀酰亚胺酯(CFSE)标记法检测B细胞增殖。结果 SQFZ减少Cy处理小鼠体质量的下降,促进胸腺指数、脾指数、MLN和PP中B细胞数量恢复,但未见明显改善骨髓抑制状态。体外实验结果表明SQFZ可以增加小鼠淋巴细胞活性,提高小鼠B细胞活化和增殖比例。结论 SQFZ促进Cy处理后小鼠GALT中B细胞数量恢复,可能通过促进B细胞增殖而发挥作用。Objective To investigate the effect of Shengqifuzheng Injection （SQFZ） on the number recovery of B cells in gut-associated lymphoid tissues （GALTs） of mice receiving cyclophosphamide-based chemotherapy. Methods BALB/c mice were randomly divided into control group, cyclophosphamide （Cy） group and SQFZ group. Mice in Cy group and SQFZ group were injected intraperitoneally with Cy （100 mg/kg）, while the control mice were injected with an equal volume of normal saline. Twenty-four hours later, mice in SQFZ group were administrated intragastricly with 1 mL SQFZ once daily for 10 consecutive days, and mice in the other groups were given the same volume of normal saline. Body mass of all the mice was measured every day. Mice were killed on day 10, and the indexes of spleen and thymus were measured, Cell cycles of bone marrow cells and the percentage of B cells in lymphocytes in mesenteric lymph node （MLN） and Peyer＇s patch （PP） were detected by flow cytometry. In vitro, after being treated with SQFZ, activity of lymphocytes was evaluzed by MTT assay; expression of CD86 on B cell surface was analyzed by flow cytometry; and B cell proliferation was tested by carboxyfluorescein succinimidyl ester （CFSE）-based lymphocyte proliferation assay. Results SQFZ alleviated the loss of body mass caused by Cy and promoted the recovery of thymus indexes, spleen indexes and B cell number in MLN and PP. But it did not alleviate the bone marrow suppression of mice in this condition. In vitro, SQFZ enhanced lymphocyte activity, and improved the activation and proliferation of B cells. Conclusion SQFZ could accelerate the recovery of B cells in GALEs of mice receiving chemotherapy and it might act by promoting B cell proliferation.

关 键 词：参芪扶正注射液 肠道相关淋巴组织 B细胞 环磷酰胺 

分 类 号：R285.5[医药卫生—中药学]