系统性红斑狼疮病人EB病毒LMP-1与ISGs表达相关性  

CORRELATION BETWEEN EXPRESSION OF EPSTEIN-BARR VIRUS LATENT MEMBRANE PROTEIN 1 AND TYPE ⅠINTERFERON INDUCED GENES IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS

作　　者：韩丽[1] 张云清[1] 孙明姝[1] 辛苗苗[1] 杨堃[2] 

机构地区：[1]青岛大学附属医院风湿免疫科,山东青岛266003 [2]青岛大学附属医院中心实验室,山东青岛266003

出　　处：《青岛大学医学院学报》2016年第3期276-279,282,共5页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省科技厅优秀中青年科学家科研奖励基金项目(BS2009YY037)

摘　　要：目的了解系统性红斑狼疮（SLE）病人体内Epstein-Barr（EB）病毒潜伏膜蛋白1（LMP-1）基因、Ⅰ型干扰素诱导基因（ISGs）表达的关系及其与SLE的相关性。方法选择SLE病人68例（其中狼疮肾炎病人39例）、健康查体者（正常对照组）40例,采用实时荧光定量PCR方法检测两组外周血淋巴细胞中EB病毒LMP-1基因及ISGs mRNA表达水平,并分析二者相关性。结果 SLE组EB病毒LMP-1mRNA水平显著高于正常对照组（U=1 039.0,P〈0.05）;SLE病人LMP-1 mRNA表达与SLE疾病活动度积分呈正相关（r=0.22,P〈0.05）。SLE病人ISGs中的2′-5′寡腺甘酸样合成酶（OASL）、干扰素刺激基因15（ISG15）和淋巴细胞抗原6复合体E（LY6E）mRNA表达水平高于正常对照组（U=377.5~725.0,P〈0.05）,黏液病毒抗性蛋白1（MX1）mRNA水平两组间比较差异无显著性（P〉0.05）。肾炎组与非肾炎组ISG15mRNA表达水平差异有显著性（U=387.5,P〈0.05）。SLE病人EB病毒LMP-1与MX1、OASL、ISG15基因mRNA水平呈正相关（r=0.003~0.219,P〈0.05）,而与LY6EmRNA无显著相关（P〉0.05）。结论 EB病毒LMP-1基因可能通过激活Ⅰ型干扰素通路导致SLE的发病与病情进展。Objective To investigate the correlation between expression of latent membrane protein 1（LMP-1）and typeⅠinterferon induced genes（ISGs）of Epstein-Barr（EB）virus in patients with systemic lupus erythematosus（SLE）. Methods A total of 68 patients with SLE-including 39 with lupus nephritis-and 40 healthy controls were enrolled in this study.Real time reverse transcription polymerase chain reaction（RT-PCR）was used to test the mRNA levels of LMP-1and ISGs,and the correlation between the two expressions was analyzed. Results In SLE group,the level of LMP-1mRNA was higher than that in the control group（U=1 039.0,P〈0.05）,the expression of LMP-1mRNA in SLE patients was positively correlated with the disease activity score（r=0.22,P〈0.05）.In SLE group,the expression levels of OASL,ISG15 and LY6Ewere higher than that in the normal control group（U=377.5-725.0,P〈0.05）,while the difference in MX1 mRNA level between the two groups was not significant（P〉0.05）.The difference in ISG15 mRNA expression between patients with lupus nephritis and without was significant（U=387.5,P〈0.05）.The mRNA level of LMP-1was positively correlated with MX1,OASL and ISG15 mRNA level（r=0.003-0.219,P〈0.05）,but was not significantly correlated with LY6EmRNA（P〉0.05）. Conclusion EB virus LMP-1is likely by activating the type-1interferon pathway leading to the development and progression of systemic lupus erythematosus.

关 键 词：红斑狼疮 系统性 疱疹病毒4型 人 潜伏膜蛋白1 干扰素Ⅰ型 

分 类 号：R593.241[医药卫生—内科学]