不同磷源对米氏凯伦藻生长和碱性磷酸酶活性的影响  

Effects of the different kinds of phosphorus sources on growth and alkaline phosphatase activity(APA) of Karenia mikimotoi Hansen

作　　者：庞勇[1,2] 聂瑞[3,2] 吕颂辉[2] 

机构地区：[1]海南省海洋与渔业科学院,海口海南570125 [2]暨南大学赤潮与海洋生物学研究中心,广州广东510632 [3]海南省海洋监测预报中心,海口海南570206

出　　处：《海洋科学》2016年第4期59-64,共6页Marine Sciences

基　　金：国家自然科学基金(U0733006);国家重点基础研究发展计划(973)项目(2010CB428702)~~

摘　　要：为了解米氏凯伦藻(Karenia mikimotoi)赤潮形成的磷营养机理,作者研究了不同磷源[三磷酸腺苷二钠盐(Adenosine disodium triphosphate,ATP)、甘油磷酸钠(sodium glycerophosphate,G-P)、卵磷脂(lecthin,LEC)和Na H2PO4·2H2O]对其生长和藻细胞碱性磷酸酶活性(alkaline phosphatase activity,APA)的影响。结果表明:(1)米氏凯伦藻可以有效利用无机磷(Na H2PO4·2H2O),对有机磷源如三磷酸腺苷二钠盐(ATP)、甘油磷酸钠(G-P)也能有效利用,但不能有效利用卵磷脂(LEC);(2)米氏凯伦藻碱性磷酸酶的检测中,在起始阶段,不同磷源(ATP,G-P,LEC和Na H2PO4·2H2O)的米氏凯伦藻APA达到最大值,米氏凯伦藻的APA分别为6.0,10.5,8.0和0.4 pmol/(个·h)。随培养时间的持续,各有机磷培养基中米氏凯伦藻的APA均表现出先逐渐减小,而后增强,最后在最大值维持的趋势,而以Na H2PO4·2H2O为磷源的米氏凯伦藻的APA没有明显的增加;(3)单个细胞的米氏凯伦藻的APA位点分布明显,大致位于细胞表面。通过研究发现,米氏凯伦藻在外界环境无机磷限制的条件下,能够较好地吸收利用有机磷维持生长,印证了近年来米氏凯伦藻赤潮频繁地发生在磷限制海域的事实。To understand the mechanisms of phosphorus nutrition in blooms of Karenia mikimotoi, the studies of the growth and alkaline phosphatase activity（APA） of K. mikimotoi were undertaken using different phosphorus sources [adenosine triphosphate disodium salt（adenosine disodium triphosphate, and ATP）, glycerophosphate（sodium glycerophosphate and G-P）, lecithin（lecthin and LEC） and Na H2PO4·2H2O]. The results revealed the following（1） K. mikimotoi could utilize PO4-P, sodium glycerophosphate（G-P）, sodium glycerophosphate（G-P）, and adenosine triphosphate（ATP） as phosphorus sources, but it could not utilize lecithin（LEC）;（2） when the APA of K. mikimotoi was assayed at the initial stage, the maximum APA in the different phosphorus sources, ATP, G-P, LEC and Na H2PO4·2H2O, of K. mikimotoiwere 6.0, 10.5, 8.0 and 0.4 pmol/（cell·h）, respectively. As the incubation time was increased, the APA decreased and then increased and maintained the maximum level. The phosphorus source of Na H2PO4·2H2O, the APA of K. mikimotoi did not increase significantly;（3） the APA site of a single K. mikimotoi cell was widely distributed, largely on the cell surface. In this research, it was found that K. mikimotoi could better absorb and utilize organic phosphorus to maintain growth in the external environment under inorganic phosphorus-limited conditions, which confirmed that the bloom of K. mikimotoi frequently occurred in phosphorus-restricted areas in recent years.

关 键 词：米氏凯伦藻(Karenia mikimotoi Hansen) 有机磷 碱性磷酸酶 

分 类 号：X173[环境科学与工程—环境科学]