纳米氧化铈对小鼠辐射损伤防护作用的研究  

Protective Effect of Cerium Oxide Nanoparticles on Mice Induced by γ-rays

作　　者：王春燕[1] 李宁[1] 佟鹏[1] 苟巧[1] 田梅[1] 邵帅[1] 李辰[1] 曲功霖[1] 齐雪松[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所毒理学研究室,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《中国辐射卫生》2016年第3期273-276,共4页Chinese Journal of Radiological Health

摘　　要：目的探讨纳米氧化铈(CeO_2)对小鼠辐射损伤的防护作用。方法 140只BALB/c小鼠随机分为正常对照组,照射对照组,阳性药物对照(氨磷汀)、纳米氧化铈低剂量组(100 mg/kg)、纳米氧化铈中剂量组(300 mg/kg)和纳米氧化铈高剂量(900 mg/kg)组。小鼠经3.5 Gy^(60)Coγ射线一次性全身照射(剂量率1 Gy/min)。于照射后3天、8天处死小鼠,检测脾脏和胸腺系数和骨髓嗜多染红细胞微核率,并对照后8天小鼠血浆SOD和小鼠胸腺淋巴细胞肌动蛋白微丝骨架进行观测。结果照后3天,与照射对照组相比,纳米氧化铈中、高剂量组的胸腺系数明显增加(P<0.05或P<0.01),优于阳性药物组;纳米氧化铈用药组脾脏系数有升高的趋势,但差异无统计学意义;纳米氧化铈药物组骨髓嗜多染红细胞微核细胞率均降低,特别是低剂量组微核细胞率与照射对照组相比差异有统计学意义(P<0.05),作用优于阳性药物组。照后8天,与照射对照组相比,纳米氧化铈药物组骨髓嗜多染红细胞微核细胞率持续降低;纳米氧化铈中剂量组小鼠血清总SOD活力有升高趋势,差异无统计学意义,胸腺淋巴细胞微丝骨架形态较完整。结论纳米氧化铈具有一定的辐射防护作用,其机制可能与提高机体免疫能力、保护造血组织免于辐射诱发的损伤有关。Objective To investigate the protective effect of cerium oxide（CeO2） nanoparticles on mice induced by γ-rays.Methods 140 BALB / c mice were randomly divided into normal control group,irradiation control group,positive control group treated with amifostine,low dose group treated with 100 mg / kg of CeO2 nanoparticles,medium dose group treated with300 mg / kg of CeO2 nanoparticles and high dose treated with 900 mg / kg of CeO2 nanoparticles.Mice were induced by 3.5 Gy of^60 Co and dose rate was 1 Gy / min.Mice were sacrificed three days and eight days after irradiation,spleen and thymus index,micronucleus rate of bone marrow polychromatic erythrocytes were examined as well as blood serum SOD and actin cytoskeleton in thymus lymphocytoplasm eight days after irradiation.Results Three days after the irradiation,compared with the irradiation control group,the thymus index significantly increased in medium dose and high dose group（ P〈0.05 or P〈0.01）,it＇s better than that of positive control group.The spleen index increased with the increase of dose of CeO2 nanoparticles,but the difference was not statistically significant.Micronucleus rate of bone marrow polychromatic erythrocytes decreased in each CeO2 nanoparticles administration group,and the significant difference was found in low dose group（ P〈0.05）,it＇s better than that of positive control group.Eight days after the irradiation,compared with the irradiation control group,Micronucleus rate of bone marrow polychromatic erythrocytes in CeO2 nanoparticles administration groups decreased.Compared with the irradiation control group,in CeO2 nanoparticles medium dose group,there was a tendency of increase of blood serum SOD activity,but the difference was not statistically significant,and actin cytoskeleton was more clear and complete.Conclusion CeO2 nanoparticles have a certain anti-radiation activity,it may be related to the immune capacity improvement and the hematopoietic tissues protection from the damage induced by radiation.

关 键 词：氧化铈(CeO2)纳米颗粒 辐射 小鼠 防护作用 

分 类 号：R818[医药卫生—放射医学]