鳞状细胞癌抗原单抗与免放试剂盒的制备及其临床价值  

Preparation of Monoclonal Antibodies and Immunoradiometric Assay Kit Against Squamous Cell Carcinoma Antigen and its Clinical Value in Diagnosis of Cancer

作　　者：王立凯 董全文 潘学继 黄丽娟 张续 

机构地区：[1]天津市协和化学发光诊断试剂有限公司,天津300457 [2]天津市免疫分析技术企业重点实验室 [3]天津市协和医药科技集团有限公司 [4]天健生物制药天津有限公司

出　　处：《中国辐射卫生》2016年第3期277-280,共4页Chinese Journal of Radiological Health

摘　　要：目的制备鳞状细胞癌抗原(SCCA)单克隆抗体及免放分析试剂盒,并探讨其在宫颈癌、肺鳞癌临床诊断中的应用价值。方法用重组人SCCA抗原免疫小鼠,通过细胞融合杂交瘤技术制备并筛选一对SCCA单抗,研制SCCA免放试剂盒,并评价其检测灵敏度,通过检测临床血清标本来评价其敏感度和特异性。结果筛选出两株可以配对的SCCA单抗,研制的SCCA免放试剂盒检测灵敏度为0.08 ng/m L,对宫颈癌和肺鳞癌的临床测定的敏感度分布可达92.24%和89.52%,特异性为98.75%。结论本SCCA免放试剂盒灵敏度高,特异性好,可用于宫颈癌、肺鳞癌的早期临床诊断和疗效观察,可有效提高宫颈癌、肺鳞癌的早期检出率。Objective To prepare monoclonal antibodies and immunoradiometric assay kit against Squamous Cell Carcinoma Antigen（ SCCA） and study its clinical value in diagnosis of Cervical cancer and lung squamous cell carcinoma.Methods With immune BALB / c mice with recombinant SCCA antigen,monoclonal antibodies were generated through splenic cell and myeloma cell fusion and screening,and immunoradiometric assay kit for SCCA was developed with 2 paired Mc Antibodies.And its sensitivity and clinical value in diagnosis of Cervical cancer and lung squamous cell carcinoma were evaluated.Results2 paired monoclonal antibodies with high affinity and high specialty were screened and immunoradiometric assay kit for SCCA was prepared.The detected sensitivity of the kit is 0.08 ng / m L,and its clinical sensitivity for Cervical cancer and lung squamous cell carcinoma was 92.24% and 89.52% respectively,specificity was 98.75%.Conclusion This immunoradiometric assay kit for SCCA has high sensitivity and specificity in detection of Cervical cancer and lung squamous cell carcinoma and could be used in clinical diagnosis.

关 键 词：鳞状细胞癌抗原 单克隆抗体 宫颈癌 肺鳞癌 免疫放射分析 

分 类 号：R817.1[医药卫生—影像医学与核医学]