丁酸钠对小鼠结肠炎性相关细胞因子平衡的影响  

Effects of sodium butyrate on the balance of inflammation-related cytokines in colitic mice

作　　者：林雪[1] 王帆[1] 冯爵荣 赵秋[1] 陶文惠[1] 李瑾[1] 

机构地区：[1]武汉大学中南医院消化内科湖北省肠病医学临床研究中心肠病湖北省重点实验室,武汉430071

出　　处：《中国临床药理学杂志》2016年第13期1198-1201,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家青年自然科学基金资助项目(81200283)

摘　　要：目的研究丁酸钠对葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠结肠组织辅助性T细胞(Th)1、Th2和Th17类细胞因子平衡的影响。方法 80只雄性Balb/c小鼠随机分成4组:正常组、模型组、空白组、实验组,正常组饮水,另外3组给予4.5%DSS连续7 d,建立结肠炎模型。空白组给予生理盐水,实验组每日1次灌胃丁酸钠0.2 m L连续7 d。监测各组的小鼠体重、生存情况、结肠长度变化,以HE染色观察结肠组织病理改变,以酶联免疫吸附法检测小鼠结肠组织Th2类细胞因子[白细胞介素(IL)IL-4、IL-6、IL-10]、Th1类细胞因子[肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)]及Th17细胞因子(IL-17)的表达情况。结果造模第7天与正常组比较,模型组体重显著降低(P<0.001),结肠长度显著缩短(P<0.05),结肠组织IL-4、IL-6、IL-10、TNF-α、IFN-γ、IL-17表达均增高。干预后第7天与空白组比较,实验组的体重明显升高(P<0.05),结肠明显伸长(P<0.05),生存率明显增高(50%vs 70%),实验组的IL-4、IL-6、IL-10、TNF-α、IFN-γ、IL-17均较空白组明显降低:(367.89±29.83)vs(693.49±228.77)pg·m L^(-1),(256.29±34.18)vs(704.49±179.00)pg·m L^(-1),(392.44±28.70)vs(992.34±159.93)pg·m L^(-1),(361.81±63.39)vs(816.31±204.00)pg·m L^(-1),(302.43±42.59)vs(527.58±152.27)pg·m L^(-1),(250.10±36.15)vs(400.16±118.28)pg·m L^(-1)(均P<0.05);实验组各细胞因子均接近正常组水平,与正常组的比值:IL-4为1.94,IL-6为1.51,IL-10为1.62,TNF-α为1.13,IFN-γ为1.34,IL-17为1.31。病理等级评估,模型组均为Ⅱ级以上;经丁酸钠干预后,实验组明显优于空白组,2组分别有71.0%,38.5%降为Ⅱ级以下。结论丁酸钠通过促进Th1/Th2/Th17类细胞因子平衡发挥促进结肠炎缓解的作用。Objective To observe the effect of butyrate on the balance of T helper cell（ Th） one,Th2 and Th17- type cytokines in the colon of dextran sulphate sodium salt（ DSS）- induced colitic mice.Methods Eighty male Balb / c mice was randomly divided into four groups as below： normal group, model group, blank group and test group. A murine model of DSS- induced colitis was induced among model group,blank group and test group. After treatment of DSS,butyrate（ 100 mg·kg^（-1）·d^-1,0. 2 m L） was used in test group by intragastric administration once a day for 7 d. The weight,survival rate and length of colon were evaluated. The production of Th1（ interleukin- 4,IL- 6,IL- 10）,Th2[tumor necrosis factor- α（ TNF- α）; inferon- γ（ IFN- γ）,IL- 17] and Th17- type cytokines（ IL- 17） was detected by enzyme- linked immunosorbent assay. The pathological change of colon was observed by light microscope with HE staining. The pathological grade was evaluated.Results Compared with normal group,the weight of mice and the length of colon in model group were significantly decreased（ P〈 0. 05）. The expression of cytokines was increased. Compared with blank group,the weight of mice and the length of colon in test group were increased（ P〈 0. 05） and the survival rate rised（ test group： 70%,blank group：50%）. Compared with blank group, Th1, Th2 and Th17- type cytokines in test group were deceased：（ 367. 89 ± 29. 83） vs（ 693. 49 ± 228. 77） pg·m L^-1,（ 256. 29 ± 34. 18） vs（ 704. 49 ± 179. 00） pg·m L^-1,（ 392. 44 ± 28. 70） vs（ 992. 34 ± 159. 93） pg·m L^-1,（ 361. 81 ± 63. 39） vs（ 816. 31 ± 204. 00） pg·m L^-1,（ 302. 43 ± 42. 59） vs（ 527. 58 ± 152. 27） pg·m L^-1,（ 250. 10 ± 36. 15） vs（ 400. 16 ± 118. 28） pg·m L^-1（ all P 〈0. 05）. It is near to those in normal group. The ratios of IL- 4,IL- 6,IL- 10,TNF- α,IFN- γand IL- 17 between test and normal group were 1. 94,1. 51,1. 62,1. 13,1. 34 and 1. 31,respectively. The patholog

关 键 词：丁酸钠 白细胞介素-4 肿瘤坏死因子-Α 结肠炎小鼠 

分 类 号：R975[医药卫生—药品]