蝎毒多肽对胃癌SGC-7901细胞侵袭转移的作用  

Effect of scorpion venom peptide on the invasion and metastasis of gastric cancer cell line SGC-7901

作　　者：林振吕[1] 郑光威[1] 张琳[2] 郑建涛[2] 陈辉[2] 

机构地区：[1]福建医科大学附属第一医院急诊外科,福州350005 [2]福建医科大学附属第一医院胃肠外科,福州350005

出　　处：《中国临床药理学杂志》2016年第13期1206-1209,共4页The Chinese Journal of Clinical Pharmacology

基　　金：福建省卫生系统中青年骨干人才培养基金资助项目(2015-ZQN-JC-23)

摘　　要：目的研究蝎毒多肽对胃癌SGC-7901细胞侵袭转移的作用。方法设立空白组和5个不同质量浓度(蝎毒多肽5,10,20,40,80μg·m L^(-1))实验组。以噻唑蓝法检测蝎毒多肽对细胞活性和增殖的影响,以划痕实验和Transwell实验观察其对细胞侵袭及转移能力的影响,以免疫印迹法检测其对基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)的影响。结果与空白组相比,蝎毒多肽可以明显抑制胃癌SGC-7901细胞的增殖,且呈现时间-浓度依赖性(P<0.05)。2个不同质量浓度(10,20μg·m L^(-1))蝎毒多肽侵袭细胞的数量分别为(35.8±5.53),(19.3±2.13),与空白组(48.3±7.34)比较差异有统计学意义(P<0.05),表明其可抑制细胞的侵袭能力。2个浓度实验组的迁移抑制率分别为(14.7±1.23)%,(28.5±3.35)%,实验组较空白组明显降低(P<0.05)。2个不同质量浓度(10,20μg·L^(-1))蝎毒多肽作用于胃癌SGC-7901细胞12 h后,与空白组比较均可抑制细胞MMP-9和MMP-2的表达。结论蝎毒多肽通过抑制MMP-2及MMP-9的表达从而抑制胃癌SGC-7901细胞的侵袭转移。Objective To observe the effect of scorpion venom peptide on the invasion and metastasis of gastric cancer cell lines SGC- 7901.Methods One blank group and 5 concentrations of（ scorpion venom polypeptide,5,10,20,40,80 μg · m L^-1） test groups were set up. The3-（ 4,5- dimethyl- 2- thiazolyl）- 2,5- diphenyl- 2- H- tetrazolium bromide（ MTT assay） was used to detect the effects of scorpion venom peptide on the activity and proliferation of cells,while its effects on cell invasion and metastasis were observed by scratch test and Transwell test.The expressions of matrix metalloproteinase- 2（ MMP- 2） and matrix metalloproteinase- 9（ MMP- 9） were observed by Western blot.Results Compared with the blank group,the scorpion venom peptide could significantly inhibit the proliferation of gastric cancer SGC- 7901 cells and show the time- concentration correlation（ P〈0. 05）. Two concentrations of scorpion venom peptide（ 10,20 μg·m L^-1） could inhibit the invasive ability of the cell,with invasion of the cell number（ 35. 8 ±5. 53）,（ 19. 3 ± 2. 13）,respectively,compared with the blank group（ 48. 3 ± 7. 34）,the difference among the groups was statistically significant（ P〈0. 05）. It means the two test groups could inhibit themigration ability of gastric cancer cell lines SGC- 7901. Compared with blank group,the migration inhibitory rates of the two concentration（ 10,20 μg · m L^-1） of test groups were significantly lower with（ 1. 47 ± 1. 23） %,（ 28. 5 ± 3. 35） %,respectively. Compared with the blank group,those in two test groups could inhibit the expressions of MMP- 9 and MMP- 2 cells at the time of 12 hours after administration by scorpion venom peptide on gastric cancer SGC- 7901 cells（ P〈0. 05）. Conclusion The scorpion venom peptide can inhibit the invasion and metastasis of gastric cancer SGC- 7901 cells by inhibiting the expressions of MMP- 2 and MMP- 9.

关 键 词：胃癌 侵袭 转移 蝎毒多肽 

分 类 号：R979.1[医药卫生—药品]