广西人群中IL-33基因rs10975521A/T、rs1929992A/G多态性的分布特点  

Distribution characteristics of rs10975521A / T and rs1929992A / G polymorphism of IL-33 gene in Guangxi population

作　　者：郭静[1] 向阳[2] 彭友帆 韦叶生[2] 蓝艳[1] 

机构地区：[1]右江民族医学院附属医院皮肤科,百色533000 [2]右江民族医学院附属医院检验科,百色533000

出　　处：《中国免疫学杂志》2016年第6期849-852,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(81260234;81560552);广西研究生教育创新计划项目(YCSZ2015223);右江民族医学院校级课题(yy2015zd001)

摘　　要：目的:研究探讨IL-33基因rs10975521A/T、rs1929992A/G的基因多态性在广西人群中的分布特点。分析两位点的基因型及基因频率在不同种族间的分布差异。方法:采用单碱基延伸法(PCR-SEB)及DNA测序法对283例受试者IL-33基因rs10975521A/T、rs1929992A/G位点进行多态性分析。用统计学方法分析其分布频率及组间差异。结果:rs10975521A/T存在AA、AT、TT三种基因型,分布频率分别为12.7%、53.0%、34.3%。其各基因型及等位基因频率在广西人群中男女性别之间无显著差异(P>0.05)。其等位基因频率与人类基因组计划公布的欧洲人(P<0.05)、北京汉族人(P<0.05)及日本人群(P<0.01)之间均有显著差异。rs1929992A/G存在AA、AG、GG三种基因型,分布频率分别为23.9%、53.7%、13.4%。其各基因型及等位基因频率在广西地区人群中男女之间也无统计医学意义(P>0.05)。其基因型与欧洲人(P<0.01)、北京汉族人(P<0.05)和日本人(P<0.01)之间差异有统计学意义。等位基因频率与欧洲人(P<0.01)、北京汉族人(P<0.05)相比差异有统计学意义。结论:IL-33基因rs10975521A/T、rs1929992A/G的基因多态性在种族和地区间存在不同程度的差异。Objective： To study and explore the distribution characteristics of rs10975521A/T and rs1929992A/G polymorphism of the IL-33 gene in Chinese Guangxi population. To compare the frequency distribution differences of allele and genotype of two sites among different ethnic. Methods：The polymorphism of rs10975521A/T and rs1929992A/G of IL-33 gene in 283 subjects were analyzed with the methods of Single base extension （PCR-SEB） and DNA sequencing,and the distribution frequency and the differences between groups of that were analyzed statisticaly. Results：Three genotypes of AA,AT and TT were found in rs10975521A/T with the frequency distribution of 12. 7%,53. 0% and 34. 3% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population （ P〉0. 05 ） . There were significant differences of the allele frequency of rs10975521A/T in the Guangxi population compared with that in the European （P〈 0. 05）, han Chinese in Bejing （P〈 0. 05） and Japanese people （P〈 0. 01）. Three genotypes of AA,AG and GG were found in rs1929992A/G with the frequency distribution of 23. 9%,53. 7% and 13. 4% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population（P〉 0. 05）,but the differences of genotype frequency of rs1929992A/G was statistically significant compared with that in the European,han Chinese in Bejing and Japanese people （ P〈 0. 05 ） . There were significant differences of the allele frequency between Guangxi population and European （P〈 0. 01）,han Chinese in Bejing（P〈 0. 05）. Conclusion： There are different degrees of discrepancy of rs10975521A/T and rs1929992A/G polymorphism of IL-33 gene among different race and region.

关 键 词：IL-33 基因多态性 广西人群 

分 类 号：Q347[生物学—遗传学]