Th17和Treg细胞以及其相关细胞因子在慢性丙型肝炎以及丙型肝炎肝硬化病人外周血中的变化及意义  

Changes and significance of Th17 and Treg cells as well as their associated cytokines in peripheral blood of patients with chronic hepatitis C and cirrhosis

作　　者：王国戗[1] 王小方[1] 汪磊[1] 潘亚晶[1] 张磊[1] 仲华[1] 张华丽[1] 范艳茹 武艳伟[1] 林志强[1] 

机构地区：[1]新乡医学院第一附属医院检验科,卫辉453100

出　　处：《中国免疫学杂志》2016年第6期882-886,共5页Chinese Journal of Immunology

摘　　要：目的:研究Th17和Treg细胞以及其相关细胞因子在慢性丙型肝炎以及丙型肝炎肝硬化病人外周血中的变化及意义,以探讨其在慢性丙型肝炎及丙型肝炎肝硬化发病中的作用。方法:用流式细胞和ELSIA技术检测慢性丙型肝炎及丙型肝炎肝硬化病人外周血Th17和Treg细胞表达率以及IL-6、IL-10、TGF-β、IL-17血清学水平,分析上述指标在健康对照组和慢性丙型肝炎组以及肝硬化组之间的差异。结果:慢性丙型肝炎病人的Th17细胞表达率(1.33%±0.30%)以及IL-6[(8.10±2.42)ng/L]、IL-17[(16.70±4.73)ng/L]血清水平明显高于健康对照组Th17细胞表达率(1.14%±0.19%)以及IL-6[(6.70±1.72)ng/L]、IL-17[(12.29±1.88)ng/L]血清水平,P<0.05;丙型肝炎肝硬化组和慢性丙型肝炎组病人外周血的Treg细胞表达率(6.21%±0.76%,5.89%±0.85%)明显高于健康对照组的Treg细胞表达率(5.51%±0.59%),P<0.05;丙型肝炎肝硬化组病人Th17/Treg的比值(0.19±0.02)明显低于慢性丙型肝炎组(0.22±0.03)和健康对照组(0.21±0.03),P<0.05;丙型肝炎肝硬化组外周血IL-10水平[(16.21±3.76)ng/L]和TGF-β水平[(5.15±0.83)ng/L]显著高于慢性丙型肝炎组[(14.36±2.78)ng/L;(4.47±0.87)ng/L]和健康对照组[(14.01±3.01)ng/L;(4.43±0.98)ng/L],P<0.05。结论:Th17和Treg细胞以及其相关细胞因子参与了丙型肝炎慢性化和肝硬化的进程,Th17和Treg细胞以及其相关细胞因子在丙型肝炎慢性化和肝硬化的治疗和预防方面可能具有重要的意义。Objective：To study the changes and significance of Th17 and Treg cells as well as their associated cytokines in pe-ripheral blood of patients with chronic hepatitis C and cirrhosis in order to investigate their role in the pathogenesis of chronic hepatitis C and hepatitis C cirrhosis. Methods：Flow cytometry and ELSIA assay were used to detect the expression ratio of Th17 and Treg cells and the serum levels of IL-10,TGF-β,IL-6 and IL-17 in peripheral blood of healthy human and patients with chronic hepatitis C and hepatitis C cirrhosis. Than we analyzed the differences of the above detection index between the healthy and patients with chronic hepatitis C and hepatitis C cirrhosis. Results：The expression ratio of Th17 cells （1. 33%±0. 30%） in peripheral blood of patients with chronic hepatitis C and IL-6[（8. 10±2. 42）ng/L] and IL-17[（16. 70±4. 73）ng/L] serum levels were significantly higher than those in the healthy （Th17：1. 14%±0. 19%） and IL-6[（1. 72±6. 70）ng/L],IL-17[（12. 29±1. 88）ng/L],P〈0. 05;The expression ratio of Treg cells in peripheral blood of patients with hepatitis C cirrhosis and chronic hepatitis C patients was significantly higher than that in the healthy （6. 21%±0. 76%,5. 89%±0. 85% vs 5. 51%±0. 59% ）,P〈0. 05,The ratio of Th17/Treg in patients with cirrhosis was significantly lower than that in patients with chronic hepatitis C and the healthy（0. 19±0. 02 vs 0. 22±0. 03,0. 21±0. 03）,P〈0. 05;the levels of IL-1[（16. 21±3. 76）ng/L] and TGF-β[（5. 15±0. 83）ng/L] in peripheral blood of patients with hepatitis C cirrhosis were significantly higher than those in chronic hepatitis C[（14. 36±2. 78）ng/L;（4. 47±0. 87）ng/L] and the healthy[（14. 01±3. 01）ng/L;（4. 43±0. 98）ng/L）],P〈0. 05. Conclusion：Th17 and Treg cells and their related cytokines involved in the process of chronicity of hepatitis C and cirrhosis,Th17 and Treg cells and their related cytokines in the treatment and prevention of chronicity of hepatitis C

关 键 词：慢性丙型肝炎 肝硬化 TH17细胞 TREG细胞 细胞因子 

分 类 号：R4[医药卫生—临床医学]