黄瓜枯萎病菌弱毒菌株诱导后叶片内几种物质含量的变化  

CONTENT VARIATIONS OF SEVERAL PHYSIOLOGICAL AND BIOCHEMICAL SUBSTANCES IN CUCUMBER LEAVES INOCULATED WITH THE ATTENUATED STRAINS FOR Fusarium oxysporum f. sp. Cucumerinum

作　　者：巨灵君 刘永胜[1] 云兴福[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《内蒙古农业大学学报（自然科学版）》2016年第2期10-16,共7页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金项目(31160100);内蒙古自治区应用技术研究与开发项目(20110711)资助

摘　　要：以黄瓜品种"津春四号"为试验材料,用黄瓜枯萎病菌弱毒菌株(FOC-RA-5)对种子和幼苗分别进行浸种和灌根处理后测定同位叶片内单宁、阿魏酸、绿原酸、游离氨基酸含量变化,并与病情指数做相关性分析。结果表明:经弱毒菌株处理后,这四种物质含量分别于不同时期高于对照。叶片内单宁含量呈现平缓下降—升高—下降的趋势,于接种后第11d出现峰值。阿魏酸、绿原酸含量均呈现先升高后降低的趋势,并分别于接种后第7d、9d出现峰值。游离氨基酸含量呈现在一定范围内上下波动的趋势,于接种5d后、13d后呈现2个峰值。物质含量与病情指数的相关分析表明:单宁、阿魏酸、绿原酸含量与植株抗病性均为显著正相关关系,游离氨基酸含量与植株抗病性无显著相关关系。The attenuated strains（FOC -RA -5）was inoculated into the cucumber seedlings , which called ＂Jinchun No. 4＂, then the content of physiological and biochemical substances which included tannins, ferulic acid, chlorogenic acid and free amino acid of leaves at the same position were determined at the time of 1 day, 3 days,5 days,7days,9days, 11 days, 13days, 15 days later, respective- ly. and the correlation analyses with disease index were done. The results showed that the contents of these four physiological and bio- chemical substances, respectively, were significantly higher than control＇ s in different time . The content variation of tannin within a gentle decline before the increasing, then decreased, and peak appeared at 11 days after inoculation. The contents variations of ferulic acid and chlorogenic acid both increased at first and then decreased, and appeared peak at 7 days, 9 days after inoculation. Free a- mino acids presented the trend that fluctuate within a certain scope, and two peaks appeared at 5 days and 13 days . The correlation analyses between substances contents and disease index showed that the tannin,ferulic acid, chlorogenic acid contents and plant dis- ease resistance were significant positive correlation , but there was no significant correlation between free amino acid content and plant disease resistance.

关 键 词：黄瓜 枯萎病菌弱毒菌株 诱导抗性 物质含量 

分 类 号：S642.2[农业科学—蔬菜学]