结核分枝杆菌感染豚鼠接种重组结核疫苗 AEC／BC02后的超敏反应分析  

Analysis of Koch phenomenon of Mycobacterium tuberculosis-infected guinea pigs vaccinated with recombinant tuberculosis vaccine AEC/BC02

作　　者：卢锦标[1] 陈保文[1] 邓海清[1] 苏城[1] 沈小兵[1] 都伟欣[1] 杨蕾[1] 王国治[1] 徐苗[1] 

机构地区：[1]中国食品药品检定研究院结核病疫苗室,北京100050

出　　处：《中华结核和呼吸杂志》2016年第7期524-528,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的：观察MTB感染豚鼠接种重组结核疫苗AEC／BC02（由抗原Ag85b、ESAT6－CFP10和复合佐剂BC02构成，简称AEC／BC02）后是否诱发超敏反应（ Koch现象）。方法18只豚鼠（雌雄各半）皮下注射MTB，5.0×10^3 CFU／只。40 d后平均分成3组，每组6只：（1）生理盐水组：注射生理盐水0.5 ml／只；（2）AEC／BC02组：注射1人份剂量／只，即（10μg Ag85b ＋10μg ESAT6－CFP10）／0.5 ml BC02／只；（3） H37Ra组：注射H37Ra灭活全菌体1 mg／只。3组均每2天注射1针，共3针。首次免疫3周后解剖豚鼠观察肝、脾、肺脏器病变，进行脾和肺组织活菌计数；肺组织切片染色后用Image－Pro Plus软件分析其炎症反应程度。结果 H37Ra组肝、脾、肺综合病变评分为（48±26）分，低于生理盐水的（62±15）分，差异无统计学意义（t＝1.093，P＝0.300）。 AEC／BC02组综合病变评分为（36±15）分，低于生理盐水组，差异有统计学意义（t＝2.980，P＝0.014），但与H37Ra组比较差异无统计学意义（t＝1.009，P＝0.337）。 H37Ra组脾活菌数为（5.31±0.80） log10 CFU，低于生理盐水组的（5.57±0.75） log10 CFU，差异无统计学意义（t ＝1.581，P ＝0.574）。 AEC／BC02组脾活菌数为（4.64±0.64） log10 CFU，低于生理盐水组，差异有统计学意义（t＝2.306，P＝0.044），但与H37Ra组比较，差异无统计学意义（t＝1.602，P＝0.140）。 AEC／BC02组和H37Ra组肺活菌数分别为（3.71±1.01）和（3.82±1.25） log10 CFU，与生理盐水组的（4.15±0.69）log10 CFU比较，差异均无统计学意义（t＝0.881，P＝0.399；t＝0.566，P＝0.584）。 H37Ra组的肺部炎症区域面积较大，占（33.0±4.4）％，高于生理盐水组的（14.8±8.4）％（t＝4.719，P＝0.001）和AEC／BC02的（19.7±7.9）％（t＝3.616， P＝0.005），而AEC／BC02组与生理盐水组比较差异无统计学意义（t＝1.041，P＝0.322）。结论肺部炎症反�Objective To observe the Koch phenomenon of Mycobacterium tuberculosis （ MTB ）-infected guinea pigs after vaccinated with killed H 37Ra bacteria or tuberculosis vaccine candidate AEC/BC02.Methods Eighteen guinea pigs were challenged subcutaneously with 5.0 ×10^3 CFU MTB and after 40 days were divided into 3 groups （6 per group）： NS group,AEC/BC02 group and H37Ra group, which were injected intramuscularly 3 times at 1 day interval with normal saline , AEC/BC02 vaccine and killed H37Ra bacteria respectively.Three weeks after the first vaccination , all guinea pigs were sacrificed to evaluate gross pathological scores for liver , spleen and lung , bacterial loads in lung and spleen , and lung inflammation.Results The gross pathological score in H37Ra group （48 ±26） was lower than that in NS group（62 ±15）,but the difference was not significant （t=1.093,P=0.300）.The AEC/BC02 group had a significantly lower gross pathological score （36 ±15） than NS group （t=2.980, P=0.014）.No significant difference between H37Ra group and AEC/BC02 group was observed （t=1.009,P=0.337）.The spleen＆amp;nbsp;bacterial load [（5.31 ±0.80） log10 CFU]in H37Ra group was slightly lower than that in NS group [（5.57 ±0.75） log10 CFU] but the difference was not significant （t=1.581,P=0.574）.In AEC/BC02 group bacterial load in the spleen was （4.64 ±0.64） log10 CFU and significantly lower than NS group （t=2.306, P=0.044） and no significant difference between H 37Ra group and AEC/BC02 group was observed （ t=1.602,P=0.140）.Meanwhile,the lung bacterial load in AEC/BC02 group was （3.71 ±1.01） log10 CFU and in H37Ra group was （3.82 ±1.25） log10 CFU.Compared to （4.15 ±0.69） log10 CFU in the NS group,no significant differences were found （t=0.881,P=0.399; t=0.566,P=0.584, respectively）. For the lung inflammation, the inflamed areas in H37Ra group were significantly larger [（33.0 ±4.4%）] than those in both NS group [（14.8 ±8.4） %, t=4.719, P=0.001] and AEC/BC02 gro

关 键 词：分枝杆菌 结核 疫苗 超敏反应 

分 类 号：R392-33[医药卫生—免疫学]