鞣花酸对四氧嘧啶糖尿病小鼠的影响  

Effect of ellagic acid on Alloxan-induced diabetes in mice

作　　者：骆静方 魏巍[1] 乔楠[1] 朱柳凤 杨丽娜[1] 

机构地区：[1]中南大学公共卫生学院营养与食品卫生学教研室,长沙410078

出　　处：《中国糖尿病杂志》2016年第7期649-651,共3页Chinese Journal of Diabetes

基　　金：湖南省科技厅自然科学基金(11JJ6091);中南大学自由探索计划(2012QNZT132)

摘　　要：目的探讨鞣花酸对四氧嘧啶糖尿病小鼠的影响。方法 40只雄性昆明小鼠随机分为正常对照(Con)组10只和四氧嘧啶处理组30只。四氧嘧啶处理组腹腔注射2%四氧嘧啶,将造模成功的21只小鼠分为模型(Model)亚组及鞣花酸低、高剂量亚组,各7只。鞣花酸低、高剂量亚组分别以160mg/kg、320 mg/kg鞣花酸连续灌胃21 d。21 d后处死动物,取肝脏和血清,测定血清ALT,AST活性,肝脏SOD活性及MDA含量等。结果与Model亚组比较,鞣花酸高剂量亚组血糖[(29.12±7.99)vs(19.30±8.08)mmol/L],血清ALT水平[(143.15±57.16)vs(65.38±33.61)U/L]、AST水平[(156.60±39.70)vs(101.11±44.16)U/L]及MDA含量[(2.81±0.81)vs(1.62±0.41)mmol/g]均下降(P<0.05),肝脏SOD活性升高[(74.23±18.82)vs(109.94±23.33)U/mg]。结论鞣花酸有降低四氧嘧啶糖尿病小鼠血糖的作用,以320 mg/kg剂量最为明显。Objective To explore the effect of ellagic acid on Alloxan‐induced diabetes of mice.Methods 40 male KM mice were randomly divided into normal control group （Con ,n=10） and Alloxan‐induced model group （n= 30 ）. The Alloxan‐induced model group was provided with 2% Alloxan by intraperitoneal injection. 21 successful DM model mice were divided into model subgroup （M ,n=7） and two ellagic acid subgroups（low dose and high dose ,n=7 ＆#215; 2）. Two ellagic acid subgroups were lavaged with 160 mg/kg and 320 mg/kg ellagic acid respectively for 21 day. After that ,all mice were sacrificed and their blood were taken for detecting serum ALT ,AST and livers were taken for detecting protein levels , SOD activities and MDA levels. Results Compared with M subgroup ,blood glucose [（29.12 ± 7.99） vs （19.30 ± 8.08） mmol/L] ,ALT [（143.15 ± 57.16） vs （65.38 ± 33.61） U/L] ,AST [（156.60 ± 39.70） vs （101.11 ± 44.16） U/L] and MDA [（2.81 ± 0.81） vs （1.62 ± 0.41） mmol/g] levels decreased in high dose subgroup （P〈 0.05） ,while the activity of liver SOD was higher [（74.23 ± 18.82 ） vs （109.94 ± 23.33） U/mg] （P〈 0.05）. Conclusion Ellagic acid has the benefits on Alloxan‐induced diabetes in mice to reduce blood glucose ,especially in the high dose group（320 mg/kg）.

关 键 词：鞣花酸 四氧嘧啶 糖尿病 抗氧化 造模 

分 类 号：R285.5[医药卫生—中药学]