钙调素参与细菌信号3-羰基辛酰基高丝氨酸内酯对拟南芥主根生长的调控  

Involvement of calmodulin in regulation of primary root elongation by N-3-oxo-octanoyl homoserine lactone in Arabidopsis thaliana

作　　者：赵芊[1,2] 张超[1] 刘方[1,2] 贾振华[1,2] 宋水山[1,2] 

机构地区：[1]河北省科学院生物研究所,石家庄050051 [2]河北省农作物主要病害微生物控制工程技术研究中心,石家庄050051

出　　处：《植物生理学报》2016年第6期933-940,共8页Plant Physiology Journal

基　　金：国家重点基础研究发展计划项目(2015CB150600);国家自然科学基金(31270880);河北省科学院高层次人才项目(20150503 LR62-10)~~

摘　　要：3-羰基辛酰基高丝氨酸内酯(3OC8-HSL)是革兰氏阴性细菌产生的群体感应信号分子,它能够被植物感知进而促进植物根的生长。本文通过药理学、遗传学和分子生物学技术分析了钙调素(Ca M)在3OC8-HSL调控植物根生长中的作用,发现Ca M抑制剂W7和TFP能够阻断3OC8-HSL对拟南芥根长的促进效应,3OC8-HSL处理能够诱导拟南芥Ca M蛋白积累和Ca M 7个亚型基因的上调表达,Ca M各亚型基因的突变体对3OC8-HSL的促根长效应不敏感,表明Ca M参与3OC8-HSL对拟南芥主根生长的调控。N-3-oxo-octanoyl homoserine lactone（3OC8-HSL） is a primary QS signal among Gram-negative bacteria. Recent evidence shows that 3OC8-HSL is able to be perceived by plants and to promote plant root growth. In this study, the function of calmodulin（Ca M） in 3OC8-HSL-induced root elongation was analyzed by pharmacological, genetical and molecular approaches. We found that the stimulatory effect of 3OC8-HSL on primary root elongation of Arabidopsis was abolished by the Ca M antagonists N-（6-aminohexyl）-5-chloro-1-naphthalene sulfonamide（W7） and trifl uoperazine（TFP）. Western-blot analysis revealed that the concentration of Ca M protein in Arabidopsis roots increased after treatment with 10 μmol·L-1 3OC8-HSL. Results from quantitative RT-PCR demonstrated that the transcription of all seven Ca M genes in Arabidopsis genome was up-regulated in the plants treated with 3OC8-HSL. The loss-of-function mutants of each At Ca M gene（At Ca M1–At Ca M7） were insensitive to 3OC8-HSL-stimulation of primary root elongation. Taken together, our results suggested that Ca M was involved in the bacterial 3OC8-HSL signaling in plant cells.

关 键 词：钙调素 N-酰基高丝氨酸内酯 主根伸长 

分 类 号：Q943.2[生物学—植物学]