小麦TaDRLea3-2基因的克隆及胁迫诱导表达分析  

Cloning of a New Gene TaDRLea3-2 from Wheat and Its Expression under Different Stresses Treatments

作　　者：梁雅珺 于正阳[1] 强智全 杜娅[1] 朱维宁[2] 张大鹏[1] 张林生[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]西北大学生命科学学院,西安710069

出　　处：《西北植物学报》2016年第6期1091-1097,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：高等学校博士学科点专项科研基金(201202041100 33);旱区作物逆境生物学国家重点实验室资助课题(CSBA2015007)

摘　　要：胚胎发育后期丰富蛋白(late embryogenesis abundant protein,LEA蛋白)是植物体中广泛存在的一类与渗透调节相关的家族蛋白,植物受非生物胁迫会大量表达。该研究采用同源克隆技术,从干旱诱导的小麦品种‘郑引1号’(Triticum aestivum L.)中获得1个新的LEA3家族基因(TaDRLea3-2),该基因全长668bp,编码区为570bp,编码189个氨基酸。生物信息学分析表明该蛋白为亲水性蛋白,二级结构以α-螺旋为主,含有9个由11个氨基酸组成的保守结构域,为典型的LEA3蛋白,存在3个磷酸化位点,无信号肽结构域及跨膜结构域,可能定位于细胞质中;实时定量PCR结果表明,TaDRLea3-2基因受干旱、高盐、低温诱导表达,同时也受外源ABA诱导,推测TaDRLea3-2为ABA依赖型LEA3基因,以不同机制参与小麦对非生物胁迫的应答过程,为深入分析小麦LEA3家族蛋白的抗逆机制奠定了基础。Late embryogenesis abundant protein（LEA）are a suite of important family proteins found in organisms,which are generally involved in osmoregulation and are expressed abundantly when subjected to environmental stresses.In this study,we isolated a novel LEA3 gene TaDRLea3-2from wheat‘Zhengyin No.1＇.The full length of TaDRLea3-2is 668 bp,whose ORF is 570 bp,encoding 189 amino acids,and consists of 9conserved 11-mer repeating motifs;Protein prediction shows that the protein is highly hydrophilic and located in the cytoplasm,with three phosphorylation sites and has no transmembrane domain or signal peptide;Secondary structure analysis shows that the main structure is alpha helix;Homology comparison and regenerating phylogenetic tree were carried out by MEGA5.1.Expression analyses revealed that TaDRLea3-2gene was induced by drought,low-temperature,high-salt stress and also moreover,can be induced by ABA treatment.These results suggested that TaDRLea3-2is an ABA-dependent LEA3 gene,participating in the response process of wheat under the abiotic stresses in different mechanisms and can lay a foundation for elucidating LEA3 gene protection mechanism under abiotic stresses in wheat.

关 键 词：小麦 TaDRLea3-2 同源克隆 序列分析 实时定量PCR 

分 类 号：Q785[生物学—分子生物学] Q786