参慈胶囊影响人肺腺癌A549/DDP裸鼠体内多药耐药DNA损伤修复的研究  

Study on the Influence of Shenci Capsule on Lung Adenocarcinoma A549/DDP Multidrug Resistance and the Repair of DNA Damage in Nude Mice

作　　者：梁昆[1] 徐立群[1] 薛兴阳[1] 邬晓东[1] 于礼建[1] 吴迪[1] 邹莹[1] 

机构地区：[1]广州医科大学院附属肿瘤医院,广东广州510095

出　　处：《云南中医学院学报》2016年第3期1-4,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：广州市卫生局中医药科技项目(20132A011035)

摘　　要：目的探讨参慈胶囊对人肺腺癌A549/DDP裸鼠体内多药耐药的影响。方法将40只C57BL/6小鼠接种人肺腺癌A549/DDP细胞悬液建立实体瘤模型,随机分为参慈胶囊组、参慈胶囊+顺铂组、顺铂组、对照组。对照组予等量生理盐水,其余各组荷瘤小鼠均用药21d,采用FQ-PCR技术检测人肺腺癌A549/DDP肺癌组织ERCC1m RNA、RRM1m RNA、h MLH1m RNA、BRCA1m RNA的表达情况。结果参慈胶囊能够降低ERCC1、RRM1、BRCA1基因的表达,且能够增加h MLH1m RNA基因的表达。结论通过多基因调节以改善人肺腺癌A549/DDP裸鼠体内顺铂耐药状态,可能是参慈胶囊逆转肺癌多药耐药的途径之一。Objective To explore the influence of Shenci capsule on lung adenocarcinoma A549/DDP multidrug resistance in nude mice. Methods Vaccinate 40 C57BL/6 mice with lung adenocarcinoma A549/DDP cell suspension and establish solid tumor model, randomly dividing into Shenci capsule team, Shenci eapsule＋cisplatin group, cisplatin group and comparison team. Control groups are given same amount of physiological saline and other groups of tumor-bearing mice offered medication 21d, adopt FQ-PCR technology to examine expressions of lung adenocarcinoma A549/DDP lung cancer tissues ERCC1 mRNA, RRMlmRNA, hMLHlmRNA, BRCAlmRNA. Results The results could reduce the expression of ERCC1, RRM1 and BRCA1 genes, and increase the expression of hMLHlmRNA gene. Conclusion Using multi-gene regulation to improve the cisplatin resistance state of lung adenocarcinoma A549/DDP in nude mice, it may be one of the methods of Shenci cansule reversing lung cancer multidrug resistance.

关 键 词：肺癌 参慈胶囊 顺铂耐药 DNA损伤修复 

分 类 号：R285.5[医药卫生—中药学]