5-氮杂-2′-脱氧胞苷和MS-275对非小细胞肺癌细胞株生长及DNMT1蛋白表达的影响  

Effect of 5-Aza-Cd R with MS275 on growth and expression of DNMT1 protein in cell lines of non-small cell lung cancer

作　　者：刘邦卿 栾加强 孙晓林[3] 白剑[1] 宋剑非[1] 杜振宗[1] 

机构地区：[1]桂林医学院第二附属医院胸外科,541119 [2]河南胸科医院,郑州市450000 [3]桂林医学院附属医院心胸外科,541001

出　　处：《实用医学杂志》2016年第13期2105-2108,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81160273;81160296);广西自然科学基金项目(编号:2013GXNSFCB019005)

摘　　要：目的:探讨表观治疗对非小细胞肺癌(NSCLC)细胞DNMT1蛋白表达的影响及生物学作用。方法:NSCLC细胞分组治疗,CCK-8、MTT检测表观药物对NSCLC细胞的影响,Western blot检测其对NSCLC细胞DNMT1蛋白表达的影响。结果:CCK-8和MTT结果显示,表观治疗可抑制NSCLC细胞的增殖及生长;Western blot结果显示,5-氮杂-2′-脱氧胞苷和MS-275的表观治疗可降低DNMT1蛋白的表达。结论:5-氮杂-2′-脱氧胞苷和MS-275的表观治疗能有效抑制NSCLC细胞中DNMT1蛋白的表达,并且对NSCLC细胞的增殖和生长有明显抑制作用。Objective To explore the characteristics and biological functions of epigenetic treatment to DNMT1 expression in NSCLC cell lines. Methods Lung cancer cells were divided into 4 groups, and drugs with different concentrations. The effect of epigenetic treatment on NSCLC cell line was detected by CCK-8 and MTF. The effect of epigenetic treatment on the expression of DNMT1 in NSCLC cell line was detected by Western blot. Results CCK-8 and MT1~ assay showed that treatment with 5-Aza-CdR and MS-275 inhibited the proliferation of NSCLC cells. Western blot showed that treatment with 5-Aza-CdR and MS-275 inhibited the DNMT1 expression in NSCLC cells. Conclusion The expression of DNMT1 gene in the NSCLC cell lines may be suppressed effectively by epigenetic treatment, and the gene may inhibit the proliferation and growth of NSCLC cell lines.

关 键 词：非小细胞肺癌 表观治疗 DNMT1蛋白 DNA甲基化 

分 类 号：R734.2[医药卫生—肿瘤]