血小板源性生长因子下调肌浆网Ca^(2+)-ATP酶促进人气道平滑肌细胞表型转化  

Platelet derived growth factor down-regulated sarcoplasmic reticulum Ca^(2+) atpase to promote the phenotype modulation of human airway smooth muscle cells

作　　者：钟晓宏[1] 程远雄[1] 姚智慧[1] 袁雅璐[1] 曹静[1] 赖文岩[2] 蔡开灿[3] 

机构地区：[1]南方医科大学南方医院呼吸科,广州市510515 [2]南方医科大学南方医院心内科,广州市510515 [3]南方医科大学南方医院胸外科,广州市510515

出　　处：《实用医学杂志》2016年第13期2133-2137,共5页The Journal of Practical Medicine

基　　金：广东省自然科学基金资助项目(编号:s2012010009036);南方医院院长基金资助项目(编号:2014A001)

摘　　要：目的:探讨肌浆网Ca^(2+)-ATP酶(SERCA2)对人气道平滑肌细胞(cells,HASMCs)表型转化的作用及其机制。方法 :原代培养HASMCs并饥饿5 d后进行分组,分别从显微镜下检测细胞的形态变化、采用Western Blot检测a-actin、SERCA2及ERK通路蛋白的变化、CCK-8检测各组细胞的增殖状态。结果 :与对照组相比,PDGF刺激后可诱导HASMCs收缩蛋白表达减少、增殖能力增加,TSG可显著抑制其作用(P<0.01);PDGF显著抑制SERCA2并促进磷酸化ERK的表达(P<0.01),U0126显著抑制了上述效应(P<0.01)。结论 :PDGF可能通过调节肌浆网Ca^(2+)-ATP酶及ERK通路蛋白的表达诱导HASMCs表型转化。Objective To investigate of the effect and mechanisman of SERCA2 on the phenotype modulation of HASMCs. Methods HASMCs were starved for 5 days and divided into different groups,then we observed morphology change of the cells from the microscope and detected a-actin,SERCA2 and p-ERK by Western Blot,cells proliferation was observed by CCK-8 method. Results Compared with the control group,PDGF could reduce a-actin of HASMCs and increased the cells proliferation ability,TSG could significantly inhibit the effect （P 〈 0.01）, PDGF could also significantly inhibit SERCA2 protein and increased the expression p-ERK （P 〈 0.01）, while U0126 significantly inhibited the effect （P 〈 0.01 ）. Conclusion PDGF may induce HASMCs phenotype modulation through the regulation of SERCA2 and p-ERK.

关 键 词：人气道平滑肌细胞 细胞表型 肌浆网CA^2+-ATP酶 ERK通路 

分 类 号：R56[医药卫生—呼吸系统]