基于体内自组装胶束形成机制探究羊脂油炙淫羊藿对大鼠温肾助阳的增效作用  

Synergistic effect of Epimedii Folium fried with suet oil for warming kidney and enhancing yang in dosage form of self-assembled micelles

作　　者：侯健[1,2] 李杰[1,2] 孙娥[1] 贾晓斌[1,2] 

机构地区：[1]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028 [2]江苏大学药学院,江苏镇江212013

出　　处：《中国中药杂志》2016年第14期2633-2637,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81274088;81503253)

摘　　要：淫羊藿传统炮制方法为羊脂油炙,具有增强淫羊藿温肾助阳的作用。该文采用腹腔注射氢化可的松法建立大鼠肾阳虚模型,以大鼠体征,血清睾酮、皮质酮含量,组织病理学检查等为评价指标,研究在辅料羊脂油的作用下,淫羊藿总黄酮在体内自组装形成胶束后对肾阳虚大鼠的增效作用。肾阳虚大鼠灌胃淫羊藿总黄酮和总黄酮胶束后,2组大鼠与模型组相比,体征及各项生化指标趋于正常,下丘脑-垂体-肾上腺-胸腺轴功能紊乱得到有效改善。实验结果表明,淫羊藿总黄酮及其自组装胶束都具有温肾壮阳的作用,其中以炙淫羊藿增强效果为佳。证明在羊脂油作用下,淫羊藿总黄酮可在体内自组装形成胶束,改善下丘脑-垂体-肾上腺-胸腺轴及主要性腺组织睾丸的病理状况,发挥增强淫羊藿温肾助阳的功效,进一步揭示了淫羊藿炮制辅料羊脂油的增效机制。The fried method with suet oil, which can strengthen the effect of Epimedii Folium in warming kidney and enhancing yang, has been widely used in the processing of Epimedii Folium in traditional Chinese medicine. In this paper, we adopted hydrocortisone-in- duced kidney-yang deficiency rat models, and took physical signs, serum testosterone, corticosterone levels and histopathological exami- nation of rats as indictors to systematically study the synergistic effect of suet oil in warming kidney and eohancing yang of fried Epimedii Folium in the dosage form of self-assembled micelles. Drugs were given orally, and compared with model group, indicating normal physi- cal signs and biochemical indicators, and significant alleviation in disorder of hypothalamic-pituitary-adrenal-thymus （HPAT） axis sup- pression. All these experimental results indicated that both Epimedium flavonoids and its self-assembled micelles, especially the processed self-assembled micelles of Epimedii Folium, showed the significant effects in warming kidney and enhancing yang, which might be corre- lated with the regulation of the disorder of HPAT axis suppression and the improvement of major pathological conditions of testicular go- nadal tissues. This discovery can further reveal the synergistic effect and mechanism of processing material suet oil on Epimedii Folium.

关 键 词：羊脂油 淫羊藿总黄酮 自组装胶束 温肾助阳 

分 类 号：R285.5[医药卫生—中药学]