瑶族药材千年健倍半萜类成分研究  

Sesquiterpenoids from rhizome of Homalomena occulta

作　　者：叶静[1] 肖美添[1] 昝珂[2] 黄雅燕[1] 张学勤[1] 

机构地区：[1]华侨大学化工与制药工程系,福建厦门361021 [2]中国食品药品检定研究院,北京100050

出　　处：《中国中药杂志》2016年第14期2655-2659,共5页China Journal of Chinese Materia Medica

基　　金：福建省自然科学基金项目(2013J01380);华侨大学中央高校基本科研业务费项目(JB-ZR1154);泉州市科技计划重点项目(2013Z13)

摘　　要：采用硅胶柱、ODS开放柱和半制备液相等色谱方法相结合,从千年健根茎乙醇提取物中的乙酸乙酯部位分离得到12个倍半萜类化合物,根据理化性质和波谱分析,化合物结构分别鉴定为3α,7α-dihydroxy-cadin-4-ene(1),3-oxofabiaimbricatan(2),3β,4α-dihydroxy-7-epi-eudesm-11(13)-ene(3),integrifonol A(4),1β,6β-dihydroxy-7-epi-eudesm-11(13)-ene(5),4β,7β,11-enantioeudesmantriol(6),epi-guaidiol(7),oplopanone(8),(-)-1β,4β,6α-trihydroxy-eudesmane(9),2α-hydroxyhomalomenol(10),(-)-T-muurolol(11),hamalomenol A(12)。化合物1~7为首次从千年健属植物中分离得到,11~12为首次从该植物中分离得到。此外对分离的部分化合物进行了抑制脂多糖诱导的小鼠巨噬细胞RAW 264.7细胞NO释放实验,发现化合物3,5和8有良好的抑制活性,其IC50分别为6.51,3.25,7.78μmol·L^(-1)。Twelve compounds were isolated from alcohol extracts of the rhizome of Homalomena occulta by using various chromato- graphic techniques including column chromatography onsilica gel and Cls reverse-phase silica gel, and semi-preparative HPLC. Their structures were identified by physico-chemical properties and spectroscopic data analysis as 3α, 7α-dihydroxy-cadin-4-ene （1）, 3-oxo- fabiaimbricatan （ 2 ）, 3β, 4α-dihydroxy-7-epi-eudesm- 11 （ 13 ） -ene （ 3 ）, integrifonol A （ 4 ）, 1β, 6β-dihydroxy-7-epi-eudesm-11 （ 13 ） - ene （5）, 4β, 7β, 11-enantioeudesmantriol （6）, epi-guaidiol （ 7 ）, oplopanone （ 8 ）, （ - ） -1β, 4β, 6α-trihydroxy-eudesmane （9）, 2a-hydroxyhomalomenol （10）, （ - ）-T-muurolol （11） and hamalomenol A （12）. Compounds 1-7 were obtained from the genus Homa- Iomena for the first time and 11-12 were firstly reported from the species. Additionally, compounds 3, 5 and 8 displayed inhibitory effects against the lipopolysaccharide （LPS）-induced nitric oxide （NO） production in mouse macrophage RAW264. 7 cells with ICs0 values of 6. 51, 3.25, 7.78 μmol L-1 , respectively.

关 键 词：天南星科 千年健属 千年健 化学成分 抗炎 

分 类 号：R29[医药卫生—民族医学]