中药化学成分透血脑屏障研究中MDCK-pHaMDR细胞模型和操作规程的建立  

Establishment of MDCK-p Ha MDR cell model and standard operation procedure for assessing blood-brain barrier permeability of chemical components of traditional Chinese medicine

作　　者：杨雁芳[1] 吴妮[1] 杨秀伟[1] 

机构地区：[1]北京大学药学院天然药物学系天然药物及仿生药物国家重点实验室,北京100191

出　　处：《中国中药杂志》2016年第14期2706-2711,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81403068;81473321);国家"十二五"科技支撑计划项目(2011BAI07B08);北京市自然科学基金项目(7152086)

摘　　要：建立MDCK-pHaMDR细胞模型和标准操作规程,用于评价中药化学成分透血脑屏障的吸收转运。通过MDCK-pHaMDR单层细胞的电镜下形态学特征、跨膜电阻、罗丹明123的胞内蓄积和双向转运以及阳性对照药咖啡因和阿替洛尔的转运程度等指标对MDCK-pHaMDR细胞模型进行评价。MDCK-pHaMDR细胞模型具有良好的完整性、紧密性和P-gp的稳定表达,阳性药物的转运结果与文献值一致。建立的MDCK-pHaMDR细胞模型的各项指标均符合要求,可用于中药化学成分透血脑屏障的吸收转运研究。To establish MDCK-pHaMDR cell model and standard operation procedure for assessing the blood-brain barrier permea- bility of chemical components of traditional Chinese medicine. MDCK-pHaMDR cell model was evaluated by determining the morpholo- gy features, transepithelial electrical resistance, bidirectional transport and intracellular accumulation of Rhodamine 123 and the appar- ent permeability of positive control drugs caffeine and atenolol. The MDCK-pHaMDR cell model had satisfactory integrity and tightness, and stable expression of P-gp. In addition, the transport results of the positive control drugs were consistent with the reported values in literature. All the parameters tested of the MDCK-pHaMDR cell model were consistent with the requirements, so the model can be used to study the blood-brain barrier permeability of chemical components of traditional Chinese medicine.

关 键 词：MDCK-pHaMDR细胞 中药化学成分 血脑屏障 吸收转运 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]