HPLC法测定蓝蒲解毒片中3个成分含量  

Determination of Monocaffeyltartaric acid,Esculetin and Caffeic acid in Lanpu Jiedu Tablet by HPLC

作　　者：吴东[1] 胡晓莲 吴柳瑾 高萌[3] 金浩鑫[1] 陈新滔 杨世林[1,3] 龚建平[1,3] 

机构地区：[1]中药固体制剂制造技术国家工程研究中心,南昌330006 [2]南昌市中西医结合医院,南昌330006 [3]江西中医药大学,南昌330004

出　　处：《江西中医药大学学报》2016年第2期78-80,共3页Journal of Jiangxi University of Chinese Medicine

基　　金：贵州省重大专项(黔科合重大专项字[2011]6005)

摘　　要：目的：建立HPLC法同时测定蓝蒲解毒片中3个成分（单咖啡酰酒石酸、秦皮乙素和咖啡酸）的含量。方法：采用Hypersil ODS2色谱柱（250mm×4.6mm,5μm）,柱温25℃,以乙腈（A）-0.1%磷酸水溶液（B）为流动相,梯度洗脱（0~2min,A：20%;2~14min,A：20%~25%;14~16min,A：25%;16~24min,A：25%~30%）,流速1.0m L/min,检测波长336nm。结果：单咖啡酰酒石酸、秦皮乙素和咖啡酸的线形范围分别为0.0038~0.0418（r=0.9997）、0.0624~0.6864μg（r=0.9997）、0.0058~0.0638μg（r=0.9999）,平均回收率分别在102.15%、97.38%、99.45%;RSD（n=6）分别为1.21%、1.53%、1.44%。结论：该方法稳定、快速,专属性强,可用于蓝蒲解毒片中单咖啡酰酒石酸、秦皮乙素、咖啡酸的含量测定及其质量控制。Objective：To establish a RP - HPLC method for simultaneous determination of Monocaffeyltartaric acid, Esculetin and Caffeic acid in Lanpu Jiedu Tablet. Methods：The separation was performed on a Hypersil ODS2 column （250 mm×4.6 mm,5 μm） with gradi- ent elution（0 ~2min,A：20 % ;2 - 14 min,A：20 % 25 % ;14 -16 min,A：25 % ;16 -24 min,A：25 % 30 % ）using acetonitrile （A） and 0.1% phosphoric acid water （B） as mobile phase at a flow rate of 1.0mL/min. The detection wavelengths was 336 nm. Results：Linear ranges of Monocaffeyltartaric acid, Esculetin and Caffeic acid were 0. 0038- 0. 0418 μg （ r = 0. 9997 ）, 0.0624 - 0. 6864 μg （ r = 0. 9997 ）, 0. 0058 0 0. 0638 μg （ r = 0.9999 ） respectively. The average recoveries （ n = 6 ） of the three lignans were 102.15 % ,97.38 % ,99.45 % and RSDwere 1.21%, 1.53 %, 1.44 % respectively. Conclusion ：The method is sensitive, accurate and can be used to determine the contents of Monocaffeyhartaric acid, Esculetin and Caffeic acid in Lanpu Jiedu Tablet ,which may also be ap- plied as a standard for the quality control of in Lanpu Jiedu Tablet.

关 键 词：蓝蒲解毒片 单咖啡酰酒石酸 秦皮乙素 咖啡酸 

分 类 号：R284.1[医药卫生—中药学]