实验性矽肺大鼠肺组织中Th1/Th2型细胞因子动态变化  

Dynamic changes of Th1/Th2 cytokines in lung tissue of experimental silicotic rat

作　　者：暴磊[1] 刘素娜[1] 李娟[1] 王娟[1] 张红义[1] 侯建永[1] 郝长付[1] 姚武[1] 

机构地区：[1]郑州大学公共卫生学院,郑州450000

出　　处：《卫生研究》2016年第4期553-557,586,共6页Journal of Hygiene Research

基　　金：国家自然科学基金(No.81273039)

摘　　要：目的检测Th1/Th2型细胞因子在实验性矽肺大鼠肺组织中的动态表达水平。方法将SPF级成年雄性SD大鼠随机分为染尘组和对照组,每组42只。模型组采用非气管暴露法一次性注入1 m L二氧化硅悬液（100 mg/m L）建立大鼠矽肺模型,对照组气管内注入等量灭菌生理盐水。分别在染尘第1天、1周、2周、3周、6周、9周和12周各处死6只大鼠,采用酶联免疫吸附实验（ELISA）检测大鼠肺组织匀浆中肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、白介素18（IL-18）、白介素4（IL-4）、白细胞介素10（IL-10）及IFN-γ/IL-4的表达水平。结果染尘组TNF-α表达量均高于对照组（P〈0.05）;染尘组IFN-γ在染尘第1天-2周表达量高于对照组（P〈0.05）;染尘组IL-18表达量均高于对照组,差异无统计学意义;染尘组IL-4在染尘第1周表达量低于对照组（P〈0.05）,第9周及12周高于对照组（P〈0.05）;染尘组IL-10在染尘第6-9周表达量高于对照组（P〈0.05）;染尘组Th1/Th2比值随染尘时间增长而降低,染尘组Th1/Th2在染尘第1天-2周比值高于对照组（P〈0.05）。结论在矽肺的发生发展过程中细胞因子发生变化：Th1型细胞因子持续高表达,Th2型细胞因子表达量先降低后升高。Objective To determine the role of Th1 / Th2 cytokines in lung tissue of experimental silicotic rat. Methods 84 healthy SPF male SD rats were randomly divided into silica exposure group and control group. Silica suspension at 1 m L（ 100 mg / m L） was administrated once by injection of non-exposure bronchus while equal amount of saline was instilled to the rats in control group,rats were sacrificed at 1-（st） day,1-（st）,2-（th）,3-（th）,6-（th）,9-（th） and 12 -（th） week after instillation. Cytokines in lung tissue were detected by ELISA. Results Compared with healthy control,the levels of TNF-α was increased at each point in silica exposure group（ P〈0. 05）. The IFN-γ in silica exposure group was increased in 1-（st） day,1-（st） and 2-（th） week（ P〈0. 05）. There was no significant difference about IL-18 between silica exposure group and control group. The IL-4 in silica exposure group was decreased in 1-（st）week,and increased in 9thweek and 12-（th） week（ P〈0. 05）. The IL-10 in silica exposure group increased in 6-（th） and 9-（th） week（ P〈0. 05）. The ratio of Th1 / Th2 of silica exposure group was increased in 1-（st）day,1-（st）and 2-（th） week（ P〈0. 05）. Conclusion The expression of Th1 cytokines are kept at a high level,Th2 cytokines decrease firstly and increase subsequently. Differential expressions of cytokines play an important role in development of silicosis.

关 键 词：矽肺 大鼠 细胞因子 

分 类 号：R135.2[医药卫生—劳动卫生]