一株气管炎疫苗生产菌株的鉴定更名  

Identification and renaming of one strain used for tracheitis vaccine production

作　　者：石继春[1] 杜慧竟[1] 梁丽[1] 李康[1] 徐潇[1] 邢玉华[1] 陈翠萍[1] 叶强[1] 

机构地区：[1]中国食品药品检定研究院卫生部生物技术产品检定方法及其标准化重点实验室,北京100050

出　　处：《中华微生物学和免疫学杂志》2016年第6期448-452,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的：对中国医学细菌保藏管理中心库藏气管炎疫苗生产菌株重新鉴定并更名。方法同时培养库藏待检菌株CMCC（B）29108和对照用模式株DSM30007T,比较二者的形态、生理生化、脂肪酸成分等特性,对16S rRNA和rpoB基因进行系统进化分析以及DNA-DNA同源性测定。结果16S rRNA基因序列比对结果显示CMCC（B）29108与不动杆菌属（Acinetobacter）相近,与模式株鲍曼不动杆菌（Acinetobacter baumannii）DSM30007T 相似性最高,形态学特性、生理生化特性以及脂肪酸成分构成均与DSM30007T 十分相似,仅存在个别差异。基于Acinetobacter属所有成员的16S rRNA和rpoB基因的系统进化分析均显示CMCC（B）29108与DSM30007T 稳定聚类成一个独立分支,且二者的DNA-DNA同源性为100%。结论基于表型、系统进化学以及化学成分的鉴定研究结果,CMCC （B）29108为鲍曼不动杆菌种内区别于模式株的一株菌,可用于气管炎疫苗生产,建议更名为鲍曼不动杆菌（ Acinetobacter baumannii）。Objective To identify and rename one strain stored in National Center for Medicine Culture Collections （ CMCC） and used for tracheitis vaccine production. Methods The test strain CMCC （B）29108 and the type strain DSM30007T were cultured on NA medium. Characteristics in morphology, physiology, biochemistry and fatty acid profile were compared between the two strains. Phylogenetic analysis was based on 16S rRNA and rpoB gene sequences, together with the DNA-DNA hybridization assay. Results A Comparative analysis of a partial sequence of the 16S rRNA gene sequence revealed that the CMCC（ B） 29108 strain was closed to the Acinetobacter species and showed the highest similarity with the type strain Acinetobacter baumannii DSM30007T. Moreover, the CMCC（B）29108 strain was highly similar to type strain DSM30007T in morphology, physiology, biochemistry and fatty acid profile. On the phylogenetic tree based on 16S rRNA and rpoB gene of all Acinetobacter members, the CMCC（B）29108 strain steadily clustered into one independent branch only with the DSM30007 T strain with a DNA-DNA hybridization value of 100%. Conclusion The CMCC（B）29108 strain that is one of the strains used for the production of tracheitis vac-cine should be assigned to the species of Acinetobacter baumannii based on its phenotypic, phylogenetic and chemotaxonomic characteristics.

关 键 词：气管炎疫苗 菌种鉴定 鲍曼不动杆菌 卡它布朗汉姆氏菌 

分 类 号：R392-33[医药卫生—免疫学]