Klebsiella pneumoniae subsp.pneumoniae引起云南省香蕉细菌性叶斑病（英文）  

A New Bacterial Leaf Spot of Banana Caused by Klebsiella pneumoniae subsp.pneumoniae in Yunnan Province of China

作　　者：陈丽华[1] 黄敏[1] 何鹏飞[1,2] Hon-hing HO 吴毅歆[4,2] 何月秋[4,2] 

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]微生物菌种筛选与应用国家地方联合工程研究中心,昆明650217 [3]纽约州立大学生物系,美国12561 [4]云南农业大学农学与生物技术学院,昆明650201

出　　处：《植物病理学报》2016年第4期536-542,共7页Acta Phytopathologica Sinica

基　　金：Corn Industry System Program of Yunnan Province(2015KJTX002)

摘　　要：为明确一种新的香蕉(Musa sapientum)细菌性叶斑病病原菌,以云南省新平县香蕉园区发现的一种新病害为供试材料,通过分离培养、形态观察、致病性测定、生理生化试验和gyrB,16S rDNA和rpoB基因片段分析,对病原菌进行了鉴定。该病由克雷伯氏肺炎球菌肺炎亚种(Klebsiella pneumoniae subsp.pneumoniae)引起,病菌可侵染香蕉叶片、假茎和果实。茎干被病原菌侵入3 d后即可出现黑色小斑点,在接种部位附近呈上下方向蔓延趋势,7 d后茎干上出现大面积棕色坏死,内部组织褐变。本文在世界上首次报道克雷伯氏肺炎球菌肺炎亚种可侵染香蕉植株,引起香蕉细菌性叶斑病。To identify the pathogen causing a new bacterial leaf spot on banana （Musa sapientum L.） in Xin- ping County, Yunnan Province of China, the bacterium was isolated from the infected banana plant and identified by cultural and morphological studies, pathogenicity test, physiological and biochemical detection as well as sequences analyses of gyrB, 16S rDNA and rpoB genes. The results showed that the leaf spot disease was caused by Klebsiella pneumoniae subsp, pneumoniae. The pathogen could infect banana fruit, pseudo-stem and leaf. Black spots appeared on diseased pseudo-stems within 3 days after inoculation and they enlarged lengthwise in both directions, causing massive brown necrosis of the internal tissues within the pseudo-stem in 7 days. This is the first report of K. pneumoniae subsp, pneumoniae causing leaf spot of banana plants in the world.

关 键 词：香蕉 克雷伯氏肺炎球菌肺炎亚种 叶斑病 

分 类 号：S432.42[农业科学—植物病理学]