假禾谷镰刀菌LAMP快速检测方法的建立  

Loop mediated isothermal amplification assay for sensitive and rapid detection of Fusanum pseudograminearum

作　　者：史亚娟[1] 王英志[1] 王振跃[1] 袁虹霞[1] 孙炳剑[1] 陈琳琳[1] 施艳[1] 李洪连[1] 

机构地区：[1]河南农业大学植物保护学院/河南省粮食作物协同创新中心/国家小麦玉米作物学重点实验室,郑州450002

出　　处：《植物病理学报》2016年第4期566-568,共3页Acta Phytopathologica Sinica

基　　金：公益性行业科技专项(201503112);河南省农业科技创新项目(2012-04)

摘　　要：假禾谷镰刀菌（Fusarium pseudograminearum）1999年由Aoki等在澳大利亚首次发现，是引起冠腐病和赤霉病的主要病原，该病菌在世界小麦产区造成严重的经济损失，包括澳大利亚、北美、土耳其、和新西兰等地区，在澳大利亚冠腐病对小麦生产造成的损失超过了5600万美元。Scab and crown rots caused by Fusarium pseudograminearum severely impact wheat production, and thereby establishing a quick and sensitive detection method is meaningful. In this study, a loop mediated isothermal amplification （LAMP） assay was developed for detection of F. pseudograminearum. In this procedure, a set of four primers matching a total of six sequences in the EF1-A gene region of F. pseudograminearum was synthesized for the LAMP assay by using genomic DNAs extracted from the mycelia. The optimum reaction temperature and assay time were determined, and the specificity of the LAMP assay was tested. This simple and sensitive method has a potential for detection of F. pseudograminearum in samples collected from the field.

关 键 词：禾谷镰刀菌 快速检测方法 LAMP 澳大利亚 小麦生产 经济损失 冠腐病 赤霉病 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]