两种IgA肾病大鼠模型的比较  

Comparison of two rat models with IgA nephropathy

作　　者：费梅[1] 熊佩华[1] 周丽霞[1] 田寿福[1] 胡玉敏[2] 

机构地区：[1]苏州大学附属第一医院中西医结合科,江苏省215006 [2]苏州大学医学部免疫教研室

出　　处：《江苏医药》2016年第13期1435-1438,F0002,共5页Jiangsu Medical Journal

基　　金：江苏省中医药局科技项目(LZ11094);江苏省中医药局优秀中青年中医临床人才研修专项课题(YX1217)

摘　　要：目的建立并比较两种IgA肾病大鼠模型。方法 36只雄性SD大鼠随机均分为三组:A组采用口服免疫原+皮下注射四氯化碳(CCl4)和免疫佐剂+静脉注射葡萄球菌肠毒素的方法建立IgA肾病模型;B组采用口服免疫原+皮下注射CCl4+静脉注射脂多糖(LPS)的方法建模;C组为正常对照组。分别在建模第6、8和10周末观察各组24-h尿蛋白定量,第10周末处死大鼠后取动脉血检测肝、肾功能,检查肾组织病理和IgA免疫荧光。结果与C组相比,A、B组第8和10周末24-h尿蛋白定量增加(P<0.05),第10周末血尿素氮、血清肌酐水平升高,白蛋白水平降低(P<0.01),且B组较A组变化更明显(P<0.05或P<0.01)。与A组相比,B组IgA免疫荧光强度增加(P<0.01),肾脏病变加重。结论采用口服免疫原+皮下注射CCl4+静脉注射LPS的方法建立IgA肾病大鼠模型的效果更好。Objective To establish and compare two rat models with IgA nephropathy. Methods Thirty-six male SD rats were equally randomized into three groups. The IgA nephropathy model in group A was established by oral bovine serum albumin（BSA）, subcutaneous injection of CC14 and immune adjuvant, and intravenous injection of staphylococcal enterotoxin B, which in group B was established by oral BSA, subcutaneous injection of CCl4, and intravenous injection of lipopolysaccharide（LPS）. Group C was taken as the normal control. The 24-hour urine was collected in the 6th, 8th and 10th week for detecting the urine protein. The rats were sacrificed in the 10th week for biochemical testing of the arterial blood and histopathologic and IgA immunofluorescence examination of the renal tissues. Results Compared with group C, the 24-hour urine protein was increased in the 8th and 10th week（P〈0. 05）, the levels of blood urine nitrogen and serum creatinine were increased and albumin was decreased in the 10th week in groups of A and B（P〈0. 01）, which were changed more obviously in group B than those in group A（P〈0. 05 or P〈0. 01）. Compared with group A, the irnmunofluo-rescence intensity of IgA was increased（P〈0. 01） and histopathologic change of renal tissues was aggravated in group B. Conclusion The scheme of oral BSA, subcutaneous injection of CCl4, and intravenous injection of LPS is better for the establishment of rat model with IgA nephropathy.

关 键 词：IGA肾病 动物模型 

分 类 号：R692[医药卫生—泌尿科学]