含1,3,4-噻二唑硫脲类新型化合物的合成及对植物病害抗性的分析  

Synthesis and antifungal and anti-TMV activities of novel thioureas containing 1,3,4-thiadiazole

作　　者：宋海龙[1] 赵钰泽 王彩霞[1,3] 周祁[2] 刘卫群[1,2] 

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]河南农业大学生命科学学院,河南郑州450002 [3]河南农业大学理学院,河南郑州450002

出　　处：《河南农业大学学报》2016年第3期370-375,共6页Journal of Henan Agricultural University

基　　金：河南省教育厅科学技术基础研究重点项目(30600808)

摘　　要：为了得到高性能的抗植物原害化合物,以氨基硫脲为起始原料,经环化、烷基化,得中间产物5-S-烃基-2-氨基-1,3,4-噻二唑,然后和4-三氟甲基异硫氰酸苯酯反应,合成了2种新化合物(4a,4b),其化学结构表征通过红外光谱(IR),核磁共振氢谱(1H-NMR)和核磁共振碳谱(13C-NMR)的检验符合4a,4b分子表征,采用高分辨质谱(HRMS)分析了4a,4b的相对分子质量,与计算值基本相等,表明4a,4b化合物完全与设计的分子结构一致。抗菌检验结果表明,在200 mg·L^(-1)及100 mg·L^(-1)质量浓度下,化合物4a,4b对新月弯孢菌、棉枯萎镰刀菌、烟草黑胫菌和禾谷镰孢菌的抑制率均高于对照药剂三唑酮;对烟草花叶病毒(TMV)抗性测试结果显示,在500 mg·L^(-1)质量浓度下,化合物4b对TMV预防抑制率达到78.38%,比宁南霉素提高25.44%。In order to get compounds which have high-powered anti-microbial pathogens, two new N- heterocyclic thiourea compounds were synthesized by 1,3,4-thiadiazole derivatives and trifluoromethyl phenyl isothiocyanate. The chemical structure and molecular weight was confirmed by IR, 1H-NMR, 13C-NMR and HRMS. The antibacterial test showed that： compound 4a and 4b exhibited better anti- fungal activities at the concentration of 200 mg ·L^-1 and 100 mg ·L^-1 against Curvularia lunata, Cot- ton Fusarium, Wilt, Phytophthora parasitica var nieotianae and Fusarium graminearum compared with triadimefon. In the prevention and treatment of tobatco mosaic virus （TMV） , the inhibition rate of compound 4b reached 78.38% at the concentration of 500 mg·L^-1, increasing by 25.44% compared with ningnanmycin, and 4b showed a good preventive effect against TMV.

关 键 词：硫脲类化合物 烟草 合成 生物活性 

分 类 号：S432[农业科学—植物病理学]