异氟烷麻醉后老年大鼠学习／记忆功能改变与脑海马CA1区谷氨酸受体变化的关系  

The correlation between spatial learning/memory alterations with changes of glutamate receptor in hippocampal CA1 region in senior rats after exposure to isoflurane

作　　者：曲向东[1] 徐诚实[1] 曲智俊[1] 周海滨[1] 张丽娜[2] 

机构地区：[1]北京积水潭医院麻醉科,100035 [2]山东省青岛市市立医院麻醉科,266071

出　　处：《中华实验外科杂志》2016年第7期1811-1813,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察异氟烷麻醉后老年大鼠学习／记忆功能障碍模型中海马CAl区谷氨酸受体的动态变化，以探讨术后认知功能障碍（POCD）的可能机制。方法将73只老年大鼠（≥18月龄）随机分为3组：对照组（n=10，不接受迷宫训练，吸入空气但不接受异氟烷麻醉），空气吸入组（n=15，接受迷宫训练，吸入空气但不接受麻醉）以及异氟烷麻醉组（n=48，接受迷宫训练和异氟烷麻醉）。应用Morris水迷宫测试接受异氟烷麻醉的大鼠麻醉前后的迷宫成绩，并以此为依据将该组大鼠其进一步分为认知功能明显受损亚组（MIS）和认知功能无明显受损亚组（NMIS）。兴奋性谷氨酸受体转运体1（GLAST）、兴奋性谷氨酸受体转运体2（EAAT2）、N-甲基-D-天门冬氨酸受体1（NMDAR1）、N-甲基-D-天门冬氨酸受体2A／B（NMDAR2A／B）、α-氨基-3羟基-5甲基-4异恶唑受体（AMPAR）以及tau蛋白的表达水平的变化通过Western blot测定。结果接受异氟烷麻醉的大鼠学习／记忆功能明显受损的发生率为10．4％（5／48）。认知功能明显受损的大鼠GLAST的表达明显高于其余3个亚组（P〈0．05），而该组各亚组谷氨酸转运体（GLT）-1、NMDAR1、NMDAR2A／B、AMPAR以及tau蛋白的表达水平差异均无统计学意义（P〉0．05）。结论异氟烷麻醉学习／记忆功能障碍大鼠模型中海马CA1区GLAST的表达增加，但EAAT2、NMDAR1、NMDAR2A／B、AMPAR和tau蛋白等无明显变化。Objective To investigate the dynamic changes of glutamate receptor in hippocampal CA1 region of senior rat associated with isoflurane - induced spatial learning/memory impairment to explore the possible mechanism of postoperative cognitive dysfunction （POCD）. Methods 73 male Sprague - Dawley rats （ 〉 18 months） were randomly divided into three main groups： （1） control subgroup （n= 10, receiving no behavioral trial, anesthesia or air exposure） ; （2） air -exposed subgroup （n = 15, receiving behavioral trial and air exposure but not anesthesia） ; （3） isoflurane anesthesia subgroup （ n = 48, receiving both behavioral trial and anesthesia）. The isoflurane - exposed rats were further divided into a learning/ memory - impaired subgroup （MIS） and a non - learning/memory - impaired subgroup （NMIS） according to their behavioral performance, which was measured using Morris water maze （MWM）. Expression levels of Glutamate aspartate transporter （ GLAST）, Glutamate transporter - 1 （ GLT - 1 ）, N - methyl - D - aspartate receptorl （NMDAR1）, N - methyl - D - aspartate receptor 2A/B （ NMDAR2A/B ）, c~ - amino - 3 - hydroxy -5 -methyl -4 -isoxazole propionate receptor （AMPAR） and tan in hippocampus were assessed via quantitative Western blotting. Results The incidence of lemarning/memory impairment in rats receiving isoflurane anesthesia was 10. 4% （5/48）. The learning/memory - impaired subgroup displayed a significantly higher GLAST level than the other three subgroups （ P 〈 0. 05 respectively）. The expression levels of GLT - 1, NMDAR1, NMDAR2A/B and AMPAR of every subgroup were comparable. Conclusion We found an up - regulation of GLAST rather than GLT - 1, NMDAR1, NMDAR2A/B, AMPAR or tan in hippocampus of aged rats associated with isoflurane - induced learning/memory impairment.

关 键 词：谷氨酸 异氟烷 学习/记忆功能受损 Morris水迷宫 谷氨酸转运体 谷氨酸受体 

分 类 号：R651.15[医药卫生—外科学]