β2糖蛋白I(β2GPI)在卵泡液中的表达水平及其与卵泡发育的关系  

Expression level of β2-glycoprotein I in follicular fluid and the relationship between β2GPⅠ and follicular development

作　　者：王薇[1] 杨婷[1] 贾学玲[1] 张学红[1] 

机构地区：[1]兰州大学第一医院生殖医学专科医院,兰州730000

出　　处：《生殖与避孕》2016年第7期596-601,共6页Reproduction and Contraception

摘　　要：目的:探讨β2糖蛋白I(β2GPI)与卵泡发育的关系。方法:选取体外受精/卵胞质内单精子注射(IVF/ICSI)助孕治疗的卵巢功能正常者100例(NC组)、多囊卵巢综合征(PCOS)患者85例(PCOS组),卵巢储备功能低下(DOR)患者62例(DOR组),长方案促排卵,收集人绒毛膜促性腺激素(h CG)注射日血清及取卵日优势卵泡的卵泡液,应用酶联免疫吸附试验法(ELISA)测定各组卵泡液中β2GPI的水平;电化学发光法测定h CG注射日血清E2水平,并对IVF/ICSI周期卵泡液中β2GPI水平与h CG注射日血清E2水平、获卵数以及卵成熟率进行相关性分析。结果:各组卵泡液中β2GPI浓度相比均无统计学差异(P>0.05);NC组IVF/ICSI周期中卵泡液中β2GPI的水平与h CG注射日血清E2水平、获卵数及卵成熟率均呈负相关(r=-0.279,P<0.05;r=-0.243,P<0.05;r=-0.711,P<0.01)。结论:卵泡液中β2GPI的水平与卵巢功能无显著相关性,卵泡液中β2GPI的表达水平与IVF/ICSI周期中卵泡的发育存在负相关。Objective： To investigate the relationship between β2-glycoprotein I （β2GPI） concentration and follicular development. Methods： One hundred female patients with normal ovarian function （NC group）, 85 polycystic ovary syndrome （PCOS） patients （PCOS group） and 62 diminished ovarian reserve （DOR） patients （DOR group） for in vitro fertilisation/intracytoplasmic sperm injection （IVF/ICSI） were selected. Conventional long protocol was performed for ovulation induction, the serum on hCG injection day and the follicular fluid of dominant follicles on oocyte retrieval day were collected. Enzyme-linked immunosorbent assay （ELISA） was used to determine the concentration of β2GPI in follicular fluid, and electrochemiluminescence immunoassay was used to detect the E2 levels in serum. The correlations between β2GPI concentration and serum E2 levels on hCG injection day, the number of oocytes and oocyte maturation rate in IVF/ICSI cycle were analyzed. Results： The level of β2GPI between PCOS group and NC group was not statistically different （P〉0.05）, which was also not statistically different between DOR group and NC group （P〉0.05）. The level of β2GPI in follicular fluid with normal ovarian function was negatively correlated with the serum E2 level on hCG injection day, the number of oocytes and oocyte maturation rate （r=-0.279, P〈0.05; r=-0.243, P〈0.05; r=-0.711, P〈0.01）. Conclusion： There was no significant correlation between β2GPI concentration and ovarian function; the expression level of β2GPI in follicular fluid was related to follicle development in IVF/ICSI cycles.

关 键 词：β2糖蛋白I(β2GPI) 卵泡发育 颗粒细胞 增殖 凋亡 

分 类 号：R714.8[医药卫生—妇产科学]