大黄灵仙胶囊对小鼠胆结石形成及肝病理和血液生化指标的影响  

Effects of Dahuang Linxian Capsule on gallstone formation,liver pathology,and blood biochemistry in C57BL/6 mice

作　　者：吕震[1] 俞渊[2] 王兵[2] 李辉[3] 金萌[3] 王宇[1] 刘明[3] 唐乾利[1] 

机构地区：[1]右江民族医学院桂西高发病防治重点实验室,百色医学硕士研究生533000 [2]广西中医药大学第一附属医院肝胆外科,南宁530023 [3]广西中医药大学研究生学院,南宁530001

出　　处：《医学研究生学报》2016年第6期577-581,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81470198);广西壮族自治区科学研究与技术开发计划项目(桂科能14123006-3)

摘　　要：目的中药复方大黄灵仙胶囊方对防治胆结石具有明显疗效。文中构建C57BL/6小鼠胆石病模型,观察大黄灵仙胶囊对C57BL/6小鼠胆结石形成、肝病理和血液生化指标水平的影响。方法将32只SPF级C57BL/6雄性小鼠随机数字表法分为4组：正常对照组、模型组、熊去氧胆酸组和大黄灵仙胶囊组,每组8只。除正常对照组外,均给予致石饲料喂养（高脂、高胆固醇）8周,期间大黄灵仙胶囊组和熊去氧胆酸组分别给予相应药物干预处理。8周后观察小鼠一般情况并采集标本,观察小鼠成石率、生化指标、肝病理变化等。结果小鼠经8周造模用药后,模型组小鼠成石率达100%,明显高于正常对照组（P〈0.01）,UDCA组、大黄灵仙胶囊组成石率42.85%、37.5%,均显著低于模型组（P〈0.05）;溴化钾压片光谱定性分析小鼠胆结石成分可在2939、1446、1382、1056/cm胆固醇特有红外吸收峰值,并经半定量分析其胆固醇成分约93%-96%;血清生化检测熊去氧胆酸组和大黄灵仙胶囊组血清低密度脂蛋白为（0.60±0.34）、（1.02±0.62）mmol/L,三酰甘油为（0.93±0.19）、（0.82±0.22）mmol/L,总胆固醇为（4.10±0.96）、（4.36±0.86）mmol/L,总胆红素为（1.97±0.34）、（1.30±0.43）mmol/L,谷草转氨酶为（121.77±35.41）、（150.60±64.17）mmol/L,谷丙转氨酶为（115.10±64.05）、（108.00±45.30）mmol/L指标均优于模型组（2.04±0.31）、（1.31±0.31）、（5.94±1.03）、（2.90±0.50）、（362.60±96.18）、（444.50±223.10）mmol/L,差异均有统计学意义（P〈0.05）;熊去氧胆酸组、大黄灵仙胶囊组小鼠肝病理学检查发现肝细胞脂肪变性、炎症、坏死等改善优于模型组,但较正常对照仍有轻微改变。结论大黄灵仙胶囊可降低小鼠胆结石模型成石率,并改善小鼠血清生化学和肝组织形态学改变,从而达到防治胆结石的功效。Objective Dahuang Linxian Capsule （DLC） is obviously effective in the prevention and treatment of gallstones. The purpose of this study was to observe the effects of DLC on the formation of gallstones, liver pathology, and blood biochemistry in the mouse model of cholelithiasis. Methods Thirty SPF-level C57BL/ 6 male mice were randomized into four groups, normal （n = 6） , gallstone model （ n = 8 ） , UDCA control （ n = 8 ） , and DLC intervention （ n = 8）. All the animals but those of the normal group were fed with lithogenic diet for 8 weeks and meanwhile those of the UDCA control and DLC intervention groups were given intragastrically UDCA and DLC, respectively. Then, we observed the general condition, the rate of gallstone formation, biochemical indexes, and liver pathologic changes of the mice. Results At 8 weeks, gallstones were formed in all the model mice, with a success rate of 100%, significantly higher than in the normal group （P 〈0.01 ）. The gallstone formation rates were 42.85% and 37.5% in the UDAC and DLC groups, respectively, both remarkably lower than in the model mice （ P 〈 0.05 ）. Pressing potassium bromide troche spectrum qualitative analysis showed that the element of cholecystolithiasis could be absorbed at the 2939, 1446, 1382, and 1056 cm^-1cholesterol-characteristic absorption peaks, with 93 -96% of cholesterol. Serum biochemical results of the UDCA control and DLC groups showed that the level of low density lipoprotein was （0.60 ±0.34） vs （ 1.02 ±0.62） mmol/L, triglyceride （0.93 ±0.19） vs （0.82 ±0.22） mmol/L, total cholesterol （4.10 ± 0.96） vs （4.36 ± 0.86） mmol/L, total bilirubin （ 1.97 ± 0.34） vs （ 1.30 ± 0.43 ） mmol/L, aspertate aminotransferase （ 121.77 ± 35.41 ） vs （ 150.60 ± 64.17 ） mmol/L, and alanine aminotransferase （ 115.10 ± 64.05 ） vs （108.0 ± 45.30） retool/L, all significantly different from those in the model group （low density lipoprotein： [2.04 ± 0.31 ] mmol/L ; triglyceride ：

关 键 词：胆结石 小鼠 大黄灵仙胶囊 病理 生化 

分 类 号：R285.5[医药卫生—中药学]