基于文献计量的CRISPR-Cas基因组编辑技术可视化分析  

Visualization analysis of CRISPR-Cas genome editing technology based on bibliometric

作　　者：赵润州[1] 徐卸古[1] 

机构地区：[1]军事医学科学院科技部

出　　处：《医学研究生学报》2016年第6期639-644,共6页Journal of Medical Postgraduates

基　　金：全军军事类研究生资助课题(2014JY398)

摘　　要：目的从文献计量角度揭示CRISPR-Cas基因组编辑技术(CRISPR-Cas技术)的发展历史、研究现状和前沿趋势,为国内科研和临床人员提供参考。方法以Web of Science平台中SCI-Expanded数据库收录的CRISPR-Cas技术主题文献为研究对象,运用Hist Cite和Cite Space软件进行统计分析和知识图谱绘制。结果 CRISPR-Cas技术的文献量呈指数增长趋势,当前研究处于蓬勃发展时期。欧美国家占据领先地位。研究领域以微生物学、生物化学和分子生物学等为主,并逐步向其他学科扩展。早期研究集中于CRISPR-Cas技术的生物学解析,随后应用其进行基因组编辑成为主流。对哺乳动物细胞的基因组编辑、不同基因组编辑技术的对比和技术原理的探索等构成CRISPR-Cas技术的研究前沿。结论 CRISPRCas技术在短时间内取得惊人发展和广泛应用,未来有望在生命科学领域发挥更为深远的影响。Objective To reveal evolution and emerging trends of research on CRISPR-Cas genome editing technology （CRISPR-Cas technology） and provide essential reference for scientific and clinical research. Methods This study retrieved CRISPR-Cas technology documents from the SCI-Expanded database in the Web of Science. The whole datasets wee loaded into HistCite and CiteSpace for further bibliometric analysis. Results For CRISPR-Cas technology research, cumulative number of publications presented an upward trend since 2002. The global research center is located in Euramerica. Publications are mainly distributed in the subjects of Bio-X and medicine. Initial research mainly focalizes on investigating the biology function. Subsequently, research paradigm shifts to experimental science. Genome engineering in mammalian cells, comparison with traditional techniques and research of technique principle are identified as research fronts. Conclusion CRISPR-Cas technology achieves stupendous achievements and extensive applications in a short term. It will further seep into virtually all the fields of life sciences.

关 键 词：CRISPR-Cas 基因组编辑 文献计量 可视化 

分 类 号：Q789[生物学—分子生物学]